Document 0200
 DOCN  M9440200
 TI    Identification and characterization of a human herpesvirus 6 gene
       segment capable of transactivating the human immunodeficiency virus type
       1 long terminal repeat in an Sp1 binding site-dependent manner.
 DT    9404
 AU    Wang J; Jones C; Norcross M; Bohnlein E; Razzaque A; Division of Viral
       Products, Center for Biologics Evaluation and; Research, Bethesda,
       Maryland 20892.
 SO    J Virol. 1994 Mar;68(3):1706-13. Unique Identifier : AIDSLINE
       MED/94149863
 AB    The human immunodeficiency virus type 1 (HIV-1) long terminal repeat
       (LTR) is transactivated by various extracellular signals and viral
       cofactors that include human herpesviruses. These transactivators are
       capable of transactivating the HIV-1 LTR through the transactivation
       response element, NF-kappa B, or other regulatory binding elements.
       Human herpesvirus 6 (HHV-6) is a potential cofactor of HIV-1. Here, we
       report that an HHV-6 gene segment, ZVH14, which can neoplastically
       transform NIH 3T3 and human keratinocytes, is capable of transactivating
       HIV-1 LTR chloramphenicol acetyltransferase constructs in an Sp1 binding
       site-dependent manner. Transactivation increased synergistically in the
       presence of multiple Sp1 sites and was dramatically reduced by
       cotransfection with oligomers designed to form triplex structures with
       HIV-1 LTR Sp1 binding sites. HIV-1 LTR NF-kappa B sites were not
       essential for ZVH14-mediated transactivation. A putative open reading
       frame in ZVH14, B115, which may encode a highly basic peptide consisting
       of 115 amino acid residues, showed transactivation capacity similar to
       that of ZVH14. This open reading frame also transactivated the HIV-1 LTR
       in an Sp1 site-dependent fashion in African green monkey kidney cells
       and human T cells. These data suggest that HHV-6 may stimulate HIV-1
       replication via transactivation of Sp1 binding sites present in the
       HIV-1 promoter.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cell Line  Chloramphenicol
       Acetyltransferase/BIOSYNTHESIS  DNA  Genes, Viral/GENETICS  Genome,
       Viral  Herpesvirus 6, Human/*GENETICS  Human  HIV Long Terminal
       Repeat/*GENETICS  HIV-1/*GENETICS  Molecular Sequence Data  NF-kappa B
       Open Reading Frames/GENETICS  Recombinant Fusion Proteins/BIOSYNTHESIS
       Regulatory Sequences, Nucleic Acid/GENETICS  Trans-Activation (Genetics)
       Trans-Activators/*GENETICS  *Transcription Factor, Sp1  Transcription,
       Genetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).