Document 0203
 DOCN  M9440203
 TI    Genetic analysis of the human immunodeficiency virus type 1 integrase
       protein.
 DT    9404
 AU    Shin CG; Taddeo B; Haseltine WA; Farnet CM; Division of Human
       Retrovirology, Dana-Farber Cancer Institute,; Boston, Massachusetts
       02115.
 SO    J Virol. 1994 Mar;68(3):1633-42. Unique Identifier : AIDSLINE
       MED/94149854
 AB    Single-amino-acid changes in a highly conserved central region of the
       human immunodeficiency virus type 1 (HIV-1) integrase protein were
       analyzed for their effects on viral protein synthesis, virion
       morphogenesis, and viral replication. Alteration of two amino acids that
       are invariant among retroviral integrases, D116 and E152 of HIV-1, as
       well as a mutation of the highly conserved amino acid S147 blocked viral
       replication in two CD4+ human T-cell lines. Mutations of four other
       highly conserved amino acids in the region had no detectable effect on
       viral replication, whereas mutations at two positions, N117 and Y143,
       resulted in viruses with a delayed-replication phenotype. Defects in
       virion precursor polypeptide processing, virion morphology, or viral DNA
       synthesis were observed for all of the replication-defective mutants,
       indicating that changes in integrase can have pleiotropic effects on
       viral replication.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cells, Cultured  Conserved
       Sequence  DNA Mutational Analysis  DNA Nucleotidyltransferases/*GENETICS
       DNA, Viral/ANALYSIS  HIV-1/*ENZYMOLOGY/GROWTH & DEVELOPMENT/GENETICS
       Molecular Sequence Data  Morphogenesis  Point Mutation  Protein
       Processing, Post-Translational  Retroviridae Proteins/BIOSYNTHESIS
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Viral Envelope
       Proteins/*GENETICS/SECRETION  Virion/GROWTH & DEVELOPMENT  *Virus
       Integration  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).