Document 0208
 DOCN  M9440208
 TI    Translational frameshifting at the gag-pol junction of human
       immunodeficiency virus type 1 is not increased in infected T-lymphoid
       cells.
 DT    9404
 AU    Cassan M; Delaunay N; Vaquero C; Rousset JP; Institut de Genetique et
       Microbiologie, Universite Paris XI,; France.
 SO    J Virol. 1994 Mar;68(3):1501-8. Unique Identifier : AIDSLINE
       MED/94149839
 AB    A frameshift event is necessary for expression of the products of the
       pol gene in a number of retroviruses, including human immunodeficiency
       virus type 1 (HIV-1). The basic signals necessary for frameshifting
       consist of a shifty sequence in which the ribosome slips and a
       downstream stimulatory structure which can be either a stem-loop or a
       pseudoknot. In HIV-1, much attention has been paid to the frameshift
       site itself, and only recently has the role of the downstream structure
       been examined. Here we used a luciferase-based experimental system to
       analyze in vivo the cis and trans factors potentially involved in
       controlling frameshifting efficiency at the gag-pol junction of HIV-1.
       We demonstrated that high-level frameshifting is dependent on the
       presence of a palindromic region located downstream of the site where
       the frameshift event takes place. Frameshifting efficiencies were found
       to be identical in mouse fibroblasts and the natural host cells of the
       virus, i.e., CD4+ human lymphoid cells. Furthermore, no increase in
       frameshifting was observed upon virus infection. Previous observations
       have shown that viral infection leads to specific alteration of tRNAs
       involved in translation of shifty sites (D. Hatfield, Y.-X. Feng, B.J.
       Lee, A. Rein, J.G. Levin, and S. Oroszlan, Virology 173:736-742, 1989).
       The results presented here strongly suggest that these modifications do
       not affect frameshifting efficiency.
 DE    Animal  Base Sequence  Cell Line  Child  DNA, Recombinant  Gene
       Expression Regulation, Viral  Genes, gag/*GENETICS  Genes, pol/*GENETICS
       Human  HIV-1/*GENETICS  Insects/ENZYMOLOGY/GENETICS  Luciferase/GENETICS
       Molecular Sequence Data  Nucleic Acid Conformation  Reading
       Frames/*GENETICS  Support, Non-U.S. Gov't  Transfection  *Translation,
       Genetic  T4 Lymphocytes/MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).