Document 0209
 DOCN  M9440209
 TI    Subcellular distribution of human immunodeficiency virus type 1 Rev and
       colocalization of Rev with RNA splicing factors in a speckled pattern in
       the nucleoplasm.
 DT    9404
 AU    Kalland KH; Szilvay AM; Langhoff E; Haukenes G; Department of
       Microbiology and Immunology, Gade Institute,; University of Bergen,
       Norway.
 SO    J Virol. 1994 Mar;68(3):1475-85. Unique Identifier : AIDSLINE
       MED/94149836
 AB    The human immunodeficiency virus type 1 (HIV-1) Rev (regulator of virion
       protein expression) protein exemplifies a new type of
       posttranscriptional regulation. One main function of Rev is to increase
       the cytoplasmic expression of unspliced and incompletely spliced
       retroviral mRNAs from which viral structural proteins are made. In that
       way, Rev is essential in order to complete the retroviral life cycle.
       The biology of Rev in the host cell has remained elusive. In this study,
       a complex distribution of Rev in single cells was found. Rev was found
       in the cytoplasm, in a perinuclear zone, in the nucleoplasm, and in the
       nucleoli. In the nucleoplasm, Rev colocalized in a speckled pattern with
       host cell factors known to assemble on nascent transcripts. Those
       factors are involved in the processing of heterogeneous RNA to spliced
       mRNA in the nucleoplasm of all cells. The distribution of Rev was
       dependent only on Rev and host cell interactions, since neither the Rev
       target RNA nor other HIV proteins were expressed in the cells. Rev was
       found in the same subcellular compartments of cells treated for extended
       periods with cycloheximide, an inhibitor of protein synthesis. This
       finding implies that Rev shuttles continuously between cytoplasmic and
       nucleoplasmic compartments. The results suggest a potential role for Rev
       both in the RNA-splicing process and in the nucleocytoplasmic transport
       of Rev-dependent HIV mRNA.
 DE    Amino Acid Sequence  Antibodies, Monoclonal/BIOSYNTHESIS  Antibody
       Specificity  Autoantibodies  Biological Transport  *Cell
       Compartmentation  Cell Nucleus/*CHEMISTRY/ULTRASTRUCTURE
       Cycloheximide/PHARMACOLOGY  Fluorescent Antibody Technique  Gene
       Products, rev/IMMUNOLOGY/*ISOLATION & PURIF  Hela Cells  Human  HIV
       Antibodies/BIOSYNTHESIS  HIV-1/*CHEMISTRY/IMMUNOLOGY
       Interphase/PHYSIOLOGY  Molecular Sequence Data  Ribonucleoproteins,
       Small Nuclear/ISOLATION & PURIF  *RNA Splicing  RNA, Small
       Nuclear/ISOLATION & PURIF  Support, Non-U.S. Gov't  Translation,
       Genetic/DRUG EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).