Document 0243
 DOCN  M9440243
 TI    Sequence variation within the immunodominant epitope-coding region from
       the external glycoprotein of human T lymphotropic virus type II in
       isolates from Seminole Indians.
 DT    9404
 AU    Lal RB; Owen SM; Rudoph D; Levine PH; Retrovirus Diseases Branch,
       Centers for Disease Control and; Prevention, Atlanta, Georgia 30329.
 SO    J Infect Dis. 1994 Feb;169(2):407-11. Unique Identifier : AIDSLINE
       MED/94149329
 AB    Western blot analysis of 16 serum specimens from Seminole Indians
       demonstrated that 14 reacted with the type-specific recombinant epitope
       (rgp46II+) of human T lymphotropic virus type II (HTLV-II), whereas the
       remaining 2 specimens did not (rgp46II-). Both rgp46II- specimens
       demonstrated presence of HTLV-II genome by polymerase chain reaction
       analysis. Culture of 1 of these specimens demonstrated presence of type
       C retrovirus particles by electron microscopy, and p24gag antigens were
       detectable in culture supernatant. Nucleotide sequence analysis of 557
       bp in the env gene (position 5405-5961) from 2 each of the rgp46II- and
       rgp46II+ specimens demonstrated sequence conservation in the rgp46II
       epitope (K-55(162-205)). Thus, lack of immune reactivity to rgp46 is not
       due to sequence variation within this epitope. This observation suggests
       that immunodominant env epitopes may not be universally recognized.
       Therefore, specimens with p24gag and r21eenv reactivity in modified
       Western blot assays should be further tested by more sensitive
       techniques.
 DE    Amino Acid Sequence  Antigenic Determinants  Base Sequence  Comparative
       Study  DNA Primers/CHEMISTRY  HTLV-II/*IMMUNOLOGY  HTLV-II
       Antigens/*CHEMISTRY  HTLV-II Infections/*IMMUNOLOGY  Indians, North
       American  Molecular Sequence Data  Polymerase Chain Reaction
       Recombinant Proteins/IMMUNOLOGY  Sequence Alignment  Viral
       Proteins/CHEMISTRY/IMMUNOLOGY  JOURNAL ARTICLE

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