Document 0245
 DOCN  M9440245
 TI    Molecular phylogeny of the Mycobacterium avium complex demonstrates
       clinically meaningful divisions.
 DT    9404
 AU    Frothingham R; Wilson KH; Infectious Disease Section, Durham VA Medical
       Center, NC 27705.
 SO    J Infect Dis. 1994 Feb;169(2):305-12. Unique Identifier : AIDSLINE
       GENBANK/X56657
 AB    Phylogenetic analysis of nucleotide sequence data is widely used for
       viral epidemiology. To explore its use in bacterial strain
       differentiation, the variable 16S-23S rDNA internal transcribed spacer
       (ITS) in 24 clinical isolates originally identified as Mycobacterium
       avium complex (MAC) was sequenced. Three isolates had an identical
       sequence that differed greatly from the rest. They belonged to the
       recently described Mycobacterium celatum. The 21 MAC clinical isolates
       gave 6 ITS sequences, each defining a sequevar. Thirteen isolates from
       11 AIDS patients with disseminated MAC disease belonged to 2 sequevars,
       which differed in ITS sequence by 1 nucleotide. In contrast, 7
       pulmonary-source MAC isolates were genetically more diverse. They
       belonged to 4 sequevars, which differed from each other by 6-20
       nucleotides and from the disseminated disease-associated sequevars by at
       least 12 nucleotides. The single urine MAC isolate had the same sequence
       as 1 of the pulmonary isolates. Because the disseminated
       disease-associated MAC strains were distinct by ITS sequence analysis,
       it should be possible to develop a molecular assay to detect them
       directly in clinical specimens or in environmental samples. Molecular
       phylogeny at the strain level may be widely useful in studies of
       bacterial epidemiology and virulence.
 DE    Adult  Base Sequence  Comparative Study  DNA, Bacterial/GENETICS  DNA,
       Ribosomal/*GENETICS  Female  Human  Male  Middle Age  Molecular Sequence
       Data  Mycobacterium avium Complex/*CLASSIFICATION/GENETICS
       Mycobacterium avium-intracellulare Infection/*MICROBIOLOGY  Phylogeny
       Sequence Alignment  Sequence Homology, Nucleic Acid  Support, U.S.
       Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).