Document 0248
 DOCN  M9440248
 TI    Characterization of human immunodeficiency virus type 1 isolates from
       children in Romania: identification of a new envelope subtype.
 DT    9404
 AU    Dumitrescu O; Kalish ML; Kliks SC; Bandea CI; Levy JA; School of
       Medicine, University of California, San Francisco; 94143-0128.
 SO    J Infect Dis. 1994 Feb;169(2):281-8. Unique Identifier : AIDSLINE
       MED/94149311
 AB    Human immunodeficiency virus type 1 (HIV-1) isolates recovered from
       infected children in Romania were characterized for their biologic,
       serologic, and molecular properties. The isolates were from subjects in
       different clinical states, and all showed cytopathic properties in
       peripheral blood mononuclear cells and varying kinetics of replication.
       The isolates grew to varying titers in macrophages and established T
       cell lines. Serologic evaluation with Romanian sera indicated stronger
       antibody response to the gp120 of Romanian isolates than to the envelope
       protein of HIV-1 isolates from other countries. Although there was
       cross-neutralization among the Romanian isolates, no substantial
       activity was noted against HIV-1 prototype strains from the United
       States, Africa, and Thailand. Genetic analysis of the envelope C2-V3
       region strongly suggests that the Romanian isolates are a subtype
       distinct from those assigned to other HIV-1 strains analyzed to date.
       This finding raises questions about the origin of HIV-1 in Romania.
 DE    Amino Acid Sequence  Child, Preschool  Consensus Sequence  Female  Human
       HIV Envelope Protein gp120/CHEMISTRY/*IMMUNOLOGY  HIV
       Infections/*EPIDEMIOLOGY/MICROBIOLOGY  HIV-1/*CLASSIFICATION/GROWTH &
       DEVELOPMENT/IMMUNOLOGY  Infant  Male  Molecular Sequence Data
       Neutralization Tests  Phylogeny  Romania  Sequence Alignment  Sequence
       Homology, Amino Acid  Support, U.S. Gov't, P.H.S.  Virus Replication
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).