Document 0252
 DOCN  M9440252
 TI    Heat-shock proteins expressed on the surface of human T cell leukemia
       virus type I-infected cell lines induce autoantibodies in rabbits.
 DT    9404
 AU    Chouchane L; Bowers FS; Sawasdikosol S; Simpson RM; Kindt TJ; Laboratory
       of Immunogenetics, National Institute of Allergy and; Infectious
       Diseases, National Institutes of Health, Rockville,; Maryland 20852.
 SO    J Infect Dis. 1994 Feb;169(2):253-9. Unique Identifier : AIDSLINE
       MED/94149307
 AB    Eight human T cell leukemia virus type I (HTLV-I)-infected cell lines
       were derived in vitro from peripheral blood mononuclear cells of 8
       rabbits. Each rabbit was then inoculated with its own HTLV-I-transformed
       cells, after which all but 1 rabbit had anti-heat-shock protein (hsp)
       antibodies in sera. Cell line RH/K34, which failed to raise a response
       to hsp70, caused lethal leukemia when > 2 x 10(8) live cells were
       injected into unrelated outbred rabbits. Rabbits injected with cell-free
       virus isolated from RH/K34 cells produced anti-hsp70 antibodies and
       became infected but developed no fatal disease. ELISA inhibition and
       flow cytometry analyses indicated that hsp molecules are expressed on
       the surface of RH/K34 and RH/K30, a nonlethal HTLV-I cell line used for
       comparison; surface hsp expression does not occur normally. Two proteins
       of approximately 72 and 93 kDa were detected by Western blot in extracts
       of RH/K30 cells. Presence of anti-hsp70 antibodies correlated with
       resistance to lethal doses of live RH/K34 cells, suggesting that hsp
       immunity may influence the outcome of RH/K34 pathogenicity.
 DE    Amino Acid Sequence  Animal  Antigenic Determinants/IMMUNOLOGY
       Antigens, Surface/*METABOLISM  Autoantibodies/*IMMUNOLOGY  Cell
       Transformation, Viral  Heat-Shock
       Proteins/CHEMISTRY/*IMMUNOLOGY/METABOLISM  HTLV
       Infections/*IMMUNOLOGY/METABOLISM  Molecular Sequence Data  Molecular
       Weight  Peptides/CHEMISTRY/IMMUNOLOGY  Rabbits  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).