Document 0264
 DOCN  M9440264
 TI    Binding of human immunodeficiency virus type-1 (HIV-1) to partially
       purified membrane vesicles of lymphoblastoid cell line CEM.
 DT    9404
 AU    Benzair AB; Hirsch I; Chermann JC; Unite de Recherches INSERM sur les
       Retrovirus et Maladies; Associees (U.322), Campus Universitaire de
       Luminy, Marseille,; France.
 SO    J Virol Methods. 1993 Dec 31;45(3):319-30. Unique Identifier : AIDSLINE
       MED/94149112
 AB    Binding of human immunodeficiency virus type 1 (HIV-1) to membrane of
       its target cells was studied by a quantitative and non-isotopic method
       called the viral membrane trapping method (VMTM). Membranes prepared
       from the CD4 positive lymphoblastoid cell line CEM and adsorbed to a
       solid support retained the ability to bind HIV-1. Similar results were
       obtained by Western dot blot and ELISA modification of VMTM, when
       membrane fraction was bound to nitrocellulose or polystyrene,
       respectively. In ELISA modification, viral association with 1 microgram
       of membranes coated on 96-well microplate was linear within a range of
       3.75 micrograms to 60 micrograms of p24gag protein. The use of anti-CD4
       mAbs, OKT4A and 13B8.2, identified CD4 molecule as a major HIV-1 binding
       component of membrane fraction. The procedure will allow the study of
       virus binding to this and to other possible additional receptor(s).
 DE    Antibodies, Monoclonal  Antigens, CD4/ANALYSIS/*METABOLISM  Binding,
       Competitive  Blotting, Western  Cell Membrane/*MICROBIOLOGY
       Enzyme-Linked Immunosorbent Assay  Human  HIV Core Protein p24/ANALYSIS
       HIV-1/GROWTH & DEVELOPMENT/*METABOLISM  Immunoblotting  Reverse
       Transcriptase/ANALYSIS  Sensitivity and Specificity  Tumor Cells,
       Cultured  T4 Lymphocytes/*MICROBIOLOGY  Virology/*METHODS  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).