Document 0266
 DOCN  M9440266
 TI    Polymerase chain reaction detection of human cytomegalovirus in over
       2000 blood specimens correlated with virus isolation and related to
       urinary virus excretion.
 DT    9404
 AU    Drouet E; Michelson S; Denoyel G; Colimon R; Department of Infectiology,
       Institut Pasteur, Lyon, France.
 SO    J Virol Methods. 1993 Dec 31;45(3):259-76. Unique Identifier : AIDSLINE
       MED/94149108
 AB    The polymerase chain reaction (PCR) as applied to human cytomegalovirus
       (HCMV) detection should provide a valuable tool for rapid, reliable
       diagnosis of infection, thereby allowing prompt treatment. However, to
       date the high sensitivity of this technique and the lack of
       semi-quantitative interpretation have hindered establishing its validity
       for diagnosing systemic infection. We describe a rapid, simple,
       semi-quantitative PCR technique for HCMV detection. The validity of the
       technique was tested objectively by analyzing over 2000 leukocytes
       specimens by PCR and comparing the results with virus isolation from
       urine and blood in concomitant samples in the absence of any clinical
       data. It could thus be established that this technique had a sensitivity
       and specificity of 97%. When the PCR signal corresponded to > or = 8000
       genome equivalents for 10(4) leukocytes, the predictive value for
       viremia was 86%. This semi-quantitative PCR technique should allow rapid
       diagnosis of systemic infection and provide a reliable means of
       monitoring clearance of CMV from blood during drug therapy.
 DE    Acquired Immunodeficiency Syndrome/COMPLICATIONS  Base Sequence
       Blotting, Southern  Comparative Study  Cytomegalovirus/GROWTH &
       DEVELOPMENT/GENETICS/*ISOLATION & PURIF  Cytomegalovirus
       Infections/COMPLICATIONS/*DIAGNOSIS  DNA, Viral/BLOOD/URINE  Human
       Leukocytes/MICROBIOLOGY  Liver Transplantation  Molecular Sequence Data
       Polymerase Chain Reaction/*METHODS  Sensitivity and Specificity
       Support, Non-U.S. Gov't  Urine/*MICROBIOLOGY  Viremia/*DIAGNOSIS  Virus
       Cultivation  Virus Shedding  JOURNAL ARTICLE

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