Document 0267
 DOCN  M9440267
 TI    Assessment of chemical disinfectants against human immunodeficiency
       virus: overcoming the problem of cytotoxicity and the evaluation of
       selected actives.
 DT    9404
 AU    Gordon V; Parry S; Bellamy K; Osborne R; Department of Veterinary
       Pathology, Glasgow University Veterinary; School, UK.
 SO    J Virol Methods. 1993 Dec 31;45(3):247-57. Unique Identifier : AIDSLINE
       MED/94149107
 AB    The aim of this study was to develop a standardised technique for
       assessing the virucidal activity of commercial disinfectants against
       human immunodeficiency virus (HIV). In the absence of any model
       procedure for HIV a protocol based on German DVV guidelines was
       developed. A major difficulty associated with such studies is the
       cytotoxic effect of the biocide on the target cells used in infectivity
       assays. This problem is most commonly overcome by dilution of the
       virus-disinfectant mixture, however, this requires high titre (> or =
       10(7) TCID50) virus which is difficult to achieve with HIV. We employed
       a simple washing technique which effectively removed cytotoxicity while
       retaining infectivity. Incorporated into a standard suspension test,
       this method supported by virus isolation procedures was sensitive and
       reproducible. The reliability of the procedure was confirmed by
       evaluating the efficacy of some commercially available cidals which were
       known to be cytotoxic; namely two instrument disinfectants,
       Sactimed-I-Steril, an aldehyde based product, Sactimed-I-Sinald a
       guanide/quaternary-ammonium combination, and Levermed, an alcohol based
       hand disinfectant.
 DE    Cell Line/DRUG EFFECTS  Cell Survival  Disinfectants/*PHARMACOLOGY
       *Disinfection  HIV/*DRUG EFFECTS  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

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