Document 0268
 DOCN  M9440268
 TI    Size-exclusion chromatographic determination of dextran sulfate in rat
       serum.
 DT    9404
 AU    Maderich AB; Sugita ET; Philadelphia College of Pharmacy and Science, PA
       19104-4495.
 SO    J Chromatogr. 1993 Oct 22;620(1):137-42. Unique Identifier : AIDSLINE
       MED/94149088
 AB    A sensitive and selective method for the determination of dextran
       sulfate in rat serum has been developed. The analysis is suitable for
       quantitation of the drug and for monitoring molecular mass changes
       occurring during biotransformation. Dextran sulfate is resolved from
       higher molecular mass serum components by high-performance aqueous
       size-exclusion chromatography. The method has been validated for the
       direct injection of serum. Sensitive detection is achieved by
       post-column reaction of the polyanionic drug with the dye
       1,9-dimethylmethylene blue. Components of serum which inhibit complex
       formation are separated chromatographically from dextran sulfate.
       Absorbance of the metachromatic complex is monitored at 525 nm.
 DE    Animal  Antiviral Agents/*BLOOD  *Chromatography, Gel  Chromatography,
       High Pressure Liquid/*METHODS/STATISTICS & NUMER  DATA  Dextran
       Sulfate/*BLOOD  Hydrogen-Ion Concentration  HIV-1/DRUG EFFECTS  Male
       Molecular Weight  Osmolar Concentration  Rats  Rats, Sprague-Dawley
       Sensitivity and Specificity  Spectrophotometry  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).