Document 0277
 DOCN  M9440277
 TI    Production of interleukin-1 alpha and interleukin-2 by separate,
       phenotypically different leukaemia and human T cell lymphotropic
       virus-1-transformed T cell clones.
 DT    9404
 AU    Holan V; Minowada J; Institute of Molecular Genetics, Academy of
       Sciences of the Czech; Republic, Prague.
 SO    Immunol Cell Biol. 1993 Dec;71 ( Pt 6):509-15. Unique Identifier :
       AIDSLINE MED/94148487
 AB    The relationship between interleukin-1 (IL-1) and interleukin-2 (IL-2)
       production and immunophenotype marker profiles was studied in a panel of
       29 leukaemia and human T cell lymphotropic virus-1 (HTLV-1)-transformed
       T cell lines. Culture supernatants from six of the 29 T cell lines
       tested increased IL-2 production by the MOLT-16 cell line in a manner
       similar to that of rIL-1 alpha or rIL-1 beta. The enhancing activity in
       the cell culture supernatants was inhibited by antibody against IL-1
       alpha. Anti-IL-1 beta antibody had no inhibitory effect. All the cell
       lines producing IL-1 alpha had characteristics of activated mature T
       cells. They were terminal deoxyribonucleotidyl transferase (TdT)-, CD4+,
       CD8-, HLA-DR+ and all were strongly positive for IL-2R alpha (Tac
       antigen) expression. However, none of the IL-1 alpha producing cell
       lines secreted detectable IL-2. A significant quantity of IL-2 was
       found, after stimulation with phytohaemagglutinin, in supernatants from
       nine of the 29 cell lines tested. The majority of IL-2 producing cell
       lines originated from less mature, non-activated T cells, as they were
       characterized by the expression of TdT, lack of HLA-DR antigens and >
       50% had no detectable IL-2R alpha. The results thus show that separate,
       phenotypically different leukaemia and HTLV-1-transformed T cell clones
       produce IL-1 alpha and IL-2.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Antibodies/IMMUNOLOGY  Cell Line, Transformed  Culture Media,
       Conditioned  DNA Nucleotidylexotransferase/IMMUNOLOGY  Fluorescent
       Antibody Technique  Human  HTLV-I/PHYSIOLOGY  Immunophenotyping
       Interferon-alpha/IMMUNOLOGY  Interleukin-1/ANALYSIS/*BIOSYNTHESIS
       Interleukin-2/ANALYSIS/*BIOSYNTHESIS  Lymphocyte Transformation
       Neutralization Tests  Support, Non-U.S. Gov't
       T-Lymphocytes/IMMUNOLOGY/*METABOLISM  Tumor Cells, Cultured  Tumor
       Necrosis Factor/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).