Document 0288
 DOCN  M9440288
 TI    Human T lymphocyte subpopulation and NK cell alterations in persons
       exposed to cocaine.
 DT    9404
 AU    Ruiz P; Cleary T; Nassiri M; Steele B; University of Miami School of
       Medicine, Department of Pathology,; Florida 33101.
 SO    Clin Immunol Immunopathol. 1994 Mar;70(3):245-50. Unique Identifier :
       AIDSLINE MED/94147722
 AB    Immunophenotypic changes in peripheral blood mononuclear cells were
       analyzed by flow cytometry in several patient groups positive for
       cocaine in their urine. Single- and dual-color immunofluorescence
       staining was performed to examine total numbers of NK, T, and B cells,
       as well as the coexpression of surface molecules on T cells associated
       with memory function, helper/inducer capacity, and activation status. In
       addition, levels of several serum proteins (including immunoglobulins)
       and other demographic variables were evaluated. Our results show that
       cocaine-intoxicated patients display reductions in the total percentage
       of CD4+ T cells and increases in the number of NK cells. Dramatic shifts
       within certain T cell subpopulations were also observed. In particular,
       there appeared to be a preferential stimulation of activated T cells as
       indicated by increased levels of class II+ CD4 and CD8 T cells and IL2r+
       CD4 T cells. Memory CD8+ T cell subpopulations (i.e., CD45RO+) were
       reduced in the cocaine-positive patients, whereas CD45R+/CD8+ T cells
       were accordingly increased in the same individuals. In some cases,
       direct correlation could be established between certain T cell
       percentages and cocaine levels. None of the serum protein levels
       measured appeared to be influenced by cocaine. These findings
       demonstrate that cocaine utilization is associated with variations in
       levels of certain T cell subpopulations and other immune cells. This may
       represent a disruption of particular immunologic cell networks which
       could ultimately influence host resistance to infection and malignancy.
 DE    Acute Phase Proteins/ANALYSIS  Adult  *Cocaine/URINE
       Complement/ANALYSIS  CD4-CD8 Ratio  Female  Flow Cytometry  Human
       Immunoglobulins/BLOOD  Immunologic Memory  Killer Cells,
       Natural/*IMMUNOLOGY  Male  Pregnancy  Substance
       Abuse/COMPLICATIONS/*IMMUNOLOGY  Support, Non-U.S. Gov't  T-Lymphocyte
       Subsets/IMMUNOLOGY  T4 Lymphocytes/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).