Document 0290
 DOCN  M9440290
 TI    The number of CD1a+ large low-density cells with dendritic cell features
       is increased in the peripheral blood of HIV+ patients.
 DT    9404
 AU    Ree HJ; Liau S; Yancovitz SR; Qureshi MN; Khan AA; Teplitz C; Beth
       Israel Medical Center, New York, New York 10003.
 SO    Clin Immunol Immunopathol. 1994 Mar;70(3):190-7. Unique Identifier :
       AIDSLINE MED/94147714
 AB    Employing a discontinuous Percoll gradient following Ficoll-Hypaque
       separation of peripheral blood mononuclear cells from normal subjects (n
       = 14) and patients with HIV-1 infection (n = 50), we separated a
       population of low-density cells consisting of monocytoid cells,
       lymphocytes, and some granulocytes. In cytospin preparations, less than
       5% of the monocytoid cells were positive for nonspecific esterase and
       CD14. However, CD1a was positive in 5-20% of these cells.
       Ultrastructurally, CD1a-labeled immunogold particles were demonstrated
       on the monocytoid cells which bore some features of dendritic cells.
       Flow cytometry of the low-density cells identified a subset of buoyant,
       large cell population, which excluded lymphocytes. This large
       low-density cell (LLDC) population was significantly expanded in
       patients with HIV infection and comprised 32.3 +/- 21.3% of low-density
       cells compared to 7.0 +/- 2.8% in normal subjects (P < 0.0001). Of the
       LLDC population 45.2 +/- 23.4% were CD1a+ in patients compared to 17.5
       +/- 13.3% in normal subjects (P < or = 0.0001). HLA-DR and HLA-DQ were
       coexpressed in approximately 70 and 50% of these CD1a+ LLDC,
       respectively. A simple nonculture assay method employed by us
       facilitates rapid screening of infected blood specimens for the CD1a+
       large low-density cells with dendritic cell features, which could be an
       additional parameter to monitor HIV disease progression.
 DE    Adult  Antigens, CD/*ANALYSIS  Cell Separation  Dendritic
       Cells/*IMMUNOLOGY  Female  Flow Cytometry  Human  HIV
       Seropositivity/BLOOD/*IMMUNOLOGY/PATHOLOGY  Immunoenzyme Techniques
       Leukocytes, Mononuclear/CYTOLOGY/IMMUNOLOGY  Male  Microscopy, Electron
       Middle Age  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).