Document 0312
 DOCN  M9440312
 TI    A monoclonal antibody directed to sulfatide inhibits the binding of
       human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein to
       macrophages but not their infection by the virus.
 DT    9404
 AU    Seddiki N; Ramdani A; Saffar L; Portoukalian J; Gluckman JC; Gattegno L;
       Laboratoire de Biologie Cellulaire, Faculte de Medecine; Paris-Nord,
       Bobigny, France.
 SO    Biochim Biophys Acta. 1994 Feb 22;1225(3):289-96. Unique Identifier :
       AIDSLINE MED/94146129
 AB    We show here that human immunodeficiency virus (HIV) envelope
       glycoproteins (gp160/gp120) bind to sulfatide and galactosyl ceramide.
       By immunofluorescence labeling with monoclonal antibody (mAb) A2B5,
       specific for ganglioside/sulfatide, we detect negatively charged
       glycolipids on CD4+ cells of the macrophage lineage and lymphocytes.
       Labeling of monocyte-derived macrophages (MDM) with mAb A2B5 was
       reproducibly found in 29 healthy donors, independently of the culture
       method and duration up to 11 days. The binding of the mAb to
       neuraminidase-treated MDM was unchanged relative to control cells, but
       mAb binding decreased after arylsulfatase treatment, which indicates
       that MDM membrane sulfatide is its major ligand. Preincubating MDM with
       the mAb partially (40-60%) but significantly inhibited the binding of
       HIV-1LAI radiolabeled recombinant gp160 to the cells. Similarly, the mAb
       entailed limited (32%) but significant inhibition of gp160 binding to
       cells of the monocytic U937 line but not to lymphoid CEM cells. However,
       mAb A2B5 did not inhibit the infection of CEM nor of U937 cells by
       HIV-1LAI strain, nor of MDM by monocytotropic HIV-1BaL. Thus, although
       sulfatide may be involved in the binding of HIV env glycoprotein to MDM
       or monocytic U937 cells, this does not play a significant role in HIV
       infection of these CD4+ cells.
 DE    Animal  Antibodies, Monoclonal/IMMUNOLOGY/*PHARMACOLOGY  Cattle  Gene
       Products, env/METABOLISM  Human  HIV Envelope Protein gp120/*METABOLISM
       HIV-1/*DRUG EFFECTS/METABOLISM  Iodine Radioisotopes
       Macrophages/*CHEMISTRY/IMMUNOLOGY/MICROBIOLOGY  Protein Binding/DRUG
       EFFECTS  Protein Precursors/METABOLISM  Recombinant Proteins/METABOLISM
       Sulfatides/*ANALYSIS/IMMUNOLOGY/METABOLISM  Support, Non-U.S. Gov't
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).