Document 0335
 DOCN  M9440335
 TI    Unintegrated two-long terminal repeat circular human T lymphotropic
       virus DNA accumulation during chronic HTLV infection.
 DT    9404
 AU    Kitamura K; Besansky NJ; Rudolph D; Nutman TB; Folks TM; Lal RB;
       Retrovirus Diseases Branch, Centers for Disease Control and; Prevention,
       Atlanta, Georgia 30333.
 SO    AIDS Res Hum Retroviruses. 1993 Nov;9(11):1167-72. Unique Identifier :
       AIDSLINE MED/94145752
 AB    Accumulation of unintegrated human T lymphotropic virus (HTLV) DNA was
       analyzed in long-term T cell lines infected with HTLV type I (HTLV-I) or
       type II (HTLV-II). By using a polymerase chain reaction-based assay,
       amplified products of expected size were obtained in all of the
       HTLV-I-infected (n = 7) and HTLV-II-infected (n = 8) cell lines. The
       signal intensities of the hybridizing band varied greatly among the cell
       lines and did not correlate with HTLV p24gag antigen production. Further
       analysis of HTLV-I-infected clones demonstrated considerable variability
       in the unintegrated DNA accumulation, suggesting that either the
       epigenetic status of the host cell or some environmental factor
       determines the occurrence of unintegrated DNA. The presence of lower
       levels of unintegrated DNA in most of the HTLV-infected, long-term cell
       lines presumably results in persistent noncytopathic infection.
 DE    Base Sequence  Cell Line  DNA Primers/GENETICS  DNA,
       Circular/GENETICS/METABOLISM  DNA, Viral/GENETICS/METABOLISM  Gene
       Expression  Human  HTLV-I/*GENETICS  HTLV-I
       Infections/GENETICS/METABOLISM/MICROBIOLOGY  HTLV-II/*GENETICS  HTLV-II
       Infections/GENETICS/METABOLISM/MICROBIOLOGY  Kinetics  Molecular
       Sequence Data  Polymerase Chain Reaction  *Repetitive Sequences, Nucleic
       Acid  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).