Document 0336
 DOCN  M9440336
 TI    Association of host cell surface adhesion receptors and other membrane
       proteins with HIV and SIV.
 DT    9404
 AU    Orentas RJ; Hildreth JE; Department of Pharmacology and Molecular
       Sciences, Johns Hopkins; University School of Medicine, Baltimore,
       Maryland 21205.
 SO    AIDS Res Hum Retroviruses. 1993 Nov;9(11):1157-65. Unique Identifier :
       AIDSLINE MED/94145751
 AB    We have developed a MAb-based capture assay to study the association of
       host cell membrane proteins with HIV and SIV. Class I and II MHC
       proteins were found to be associated with HIV as previously described.
       In addition to these molecules a number of other host molecules were
       found to be acquired by HIV, including CD71, CD63, CD43, and CD8. We
       have demonstrated that the major leukocyte adhesion receptors LFA-1
       (CD11A/CD18) and CD44 are also associated with HIV. The level of surface
       expression of host membrane proteins did not predict relative expression
       (capture efficiency) of the virus. The use of virus-susceptible
       indicator cells showed that the assay involved host membrane
       protein-mediated capture of infectious HIV and SIV particles. Our data
       indicate that HIV and SIV acquire a number of host membrane proteins
       including adhesion receptors and that this process may be nonrandom. The
       acquisition of host cell adhesion receptors by HIV and SIV could have
       profound effects on the biology of the viruses, including binding,
       infectivity, and tropism.
 DE    Animal  Antibodies, Monoclonal  Antigens, CD/METABOLISM  Binding Sites
       Cell Line  Histocompatibility Antigens Class I/METABOLISM  Human
       HIV/*METABOLISM/PHYSIOLOGY/PATHOGENICITY  HLA-D Antigens/METABOLISM
       Integrins/*METABOLISM  Membrane Proteins/*METABOLISM  Protein Binding
       Support, U.S. Gov't, P.H.S.  SIV/*METABOLISM/PHYSIOLOGY/PATHOGENICITY
       Virus Replication  JOURNAL ARTICLE

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