Document 0341
 DOCN  M9440341
 TI    Increased efficacy of human natural interferon alpha (IFN-alpha n3)
       versus human recombinant IFN-alpha 2 for inhibition of HIV-1 replication
       in primary human monocytes.
 DT    9404
 AU    Fan SX; Skillman DR; Liao MJ; Testa D; Meltzer MS; Department of
       Cellular Immunology, Walter Reed Army Institute of; Research,
       Washington, D.C. 20307-5100.
 SO    AIDS Res Hum Retroviruses. 1993 Nov;9(11):1115-22. Unique Identifier :
       AIDSLINE MED/94145746
 AB    Natural IFN-alpha n3, a purified mixture of many different natural IFN
       alpha species, was 10- to 100-fold more effective than equal
       concentrations of human rIFN-alpha 2b or rIFN-alpha 2a for inhibition of
       HIV replication in primary human monocytes. This difference was highly
       reproducible in multiple side-by-side experiments using the identical
       HIV-1 inoculum and the same monocyte target cells: natural IFN-alpha n3
       was more effective than rIFN-alpha 2b at lower concentrations for
       protection against a constant HIV-1 inoculum; cells treated with natural
       IFN-alpha n3 were protected against a greater HIV-1 challenge than were
       cells treated with the same concentration of rIFN-alpha 2b.
       Fractionation of natural IFN-alpha n3 by reversed-phase high-pressure
       liquid chromatography (RP-HPLC) showed that most antiviral activity for
       HIV localized to discrete and reproducible peaks. The RP-HPLC peak that
       contained purified natural IFN-alpha 2b was the least effective
       fraction. These data suggest heterogeneity among IFN-alpha species for
       antiviral activity against HIV and may provide a molecular basis for
       more effective IFN-alpha therapy.
 DE    Cells, Cultured  Comparative Study  Dose-Response Relationship, Drug
       Human  HIV-1/*DRUG EFFECTS/PHYSIOLOGY  Interferon Alfa-2a/ADMINISTRATION
       & DOSAGE/*PHARMACOLOGY  Interferon Alfa-2b/ADMINISTRATION &
       DOSAGE/*PHARMACOLOGY  Interferon-alpha/ADMINISTRATION & DOSAGE/ISOLATION
       & PURIF/  *PHARMACOLOGY  Monocytes/DRUG EFFECTS/MICROBIOLOGY  Virus
       Replication/DRUG EFFECTS  JOURNAL ARTICLE

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