Document 0342
 DOCN  M9440342
 TI    HIV gene expression enhances T cell susceptibility to hydrogen
       peroxide-induced apoptosis.
 DT    9404
 AU    Sandstrom PA; Roberts B; Folks TM; Buttke TM; Department of Microbiology
       and Immunology, East Carolina; University, School of Medicine,
       Greenville, North Carolina 27858.
 SO    AIDS Res Hum Retroviruses. 1993 Nov;9(11):1107-13. Unique Identifier :
       AIDSLINE MED/94145745
 AB    A human T cell lineage was used to determine the possible effects of HIV
       infection on T cell antioxidant status. On inoculation into serum-free
       culture, 8E5, a constitutive HIV-expressing T cell line, underwent
       apoptosis whereas cell death was not observed with the uninfected A3.01
       or latently HIV-infected 8E5L T cell lines. 8E5 survival was markedly
       prolonged by supplementing the serum-free medium with either
       A3.01-conditioned medium, catalase, vitamin E, or 2-mercaptoethanol, but
       supplementation with ascorbic acid, glutathione, or N-acetylcysteine had
       no effect. Consistent with their being in a state of oxidative stress,
       8E5 cells displayed reduced levels of catalase activity, and were more
       susceptible to killing by exogenous hydrogen peroxide (H2O2) than A3.01
       and 8E5L cells. These results demonstrate an inverse correlation between
       HIV gene expression and antioxidant status in human T cells. Enhanced
       cytotoxicity of HIV-infected, antioxidant-deficient CD4 T cells
       following exposure to H2O2 in lymphoid tissues responding to
       opportunistic pathogens may contribute to the depletion of CD4 T cells
       in AIDS.
 DE    Antioxidants/METABOLISM  Apoptosis/DRUG EFFECTS  Catalase/DEFICIENCY
       Cell Line  Gene Expression  *Genes, Viral  Human  Hydrogen
       Peroxide/PHARMACOLOGY  HIV/*GENETICS/PATHOGENICITY  Oxidation-Reduction
       Reactive Oxygen Species/METABOLISM  Stress/METABOLISM  Support, Non-U.S.
       Gov't  T4 Lymphocytes/CYTOLOGY/DRUG EFFECTS/*MICROBIOLOGY  JOURNAL
       ARTICLE

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