Document 0345
 DOCN  M9440345
 TI    Inhibition of entry of HIV into cells by poly(A).poly(U).
 DT    9404
 AU    Krust B; Callebaut C; Hovanessian AG; Unite de Virologie et Immunologie
       Cellulaire, Unite 1157 CNRS,; Institut Pasteur, Paris, France.
 SO    AIDS Res Hum Retroviruses. 1993 Nov;9(11):1087-90. Unique Identifier :
       AIDSLINE MED/94145742
 AB    Polyadenylic-polyuridylic acid referred to as poly(A).poly(U), is a
       synthetic double-stranded RNA that has been shown to manifest both
       antitumoral and immunodulatory activities. Previously, we have reported
       that poly(A).poly(U) inhibits HIV infection in cell cultures. Here we
       provide direct evidence to demonstrate that the inhibitory action of
       poly(A).poly(U) is through its capacity to prevent entry of HIV
       particles into CD4-positive T lymphocytes. Such inhibition of HIV entry
       is also observed in the case of other polyanions such as heparin,
       dextran sulfate, and poly(I).poly(C). The mechanism of inhibition
       appears to occur postbinding of HIV particles to the CD4 receptor
       molecules, because the binding of the external envelope glycoprotein of
       HIV-1 (gp120) is not affected significantly in the presence of
       poly(A).poly(U) or other polyanions. These results confirm the potential
       of poly(A).poly(U) as an antiviral drug against HIV infection.
 DE    Antiviral Agents/*PHARMACOLOGY  Binding Sites  Cell Line  Dextran
       Sulfate/PHARMACOLOGY  Heparin/PHARMACOLOGY  Human  HIV Envelope Protein
       gp120/METABOLISM  HIV-1/*DRUG EFFECTS/PHYSIOLOGY  Poly A-U/*PHARMACOLOGY
       Poly I-C/PHARMACOLOGY  Receptors, HIV/DRUG EFFECTS/PHYSIOLOGY  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).