Document 0346
 DOCN  M9440346
 TI    CD8+ T lymphocyte-mediated inhibition of HIV-1 long terminal repeat
       transcription: a novel antiviral mechanism.
 DT    9404
 AU    Chen CH; Weinhold KJ; Bartlett JA; Bolognesi DP; Greenberg ML;
       Department of Surgery, Duke University Medical Center, Durham,; North
       Carolina 27710.
 SO    AIDS Res Hum Retroviruses. 1993 Nov;9(11):1079-86. Unique Identifier :
       AIDSLINE MED/94145741
 AB    HIV-1 infection evokes a vigorous antiviral response that may
       participate in resolving the initial peak of plasma viremia and
       maintenance of the asymptomatic state. CD8+ T lymphocytes of
       HIV-1-infected individuals play a critical role in the cellular anti-HIV
       response. In agreement with previous reports, we observed a potent
       suppressive effect on HIV-1 production from autologous CD4+ T
       lymphocytes by CD8+ T lymphocytes from asymptomatic HIV-1-infected
       individuals. To elucidate the mechanism(s) of the nonlytic suppressive
       antiviral activity, we examined the effect of CD8+ T lymphocytes on the
       transcriptional activity of the HIV-1 promoter (HIV-LTR). CD8+
       lymphocytes from HIV-1-infected asymptomatic individuals suppressed
       tat-mediated HIV-LTR transcription in CD4+ lymphocytes. HIV-LTR
       transcriptional activity was suppressed by CD8 lymphocytes to an extent
       similar to tat-mediated transcription whereas CMV immediate early gene
       promoter activity was not affected. In contrast to the suppressive
       effect seen with CD8+ lymphocytes from HIV-1-infected individuals, CD8+
       lymphocytes from uninfected individuals did not significantly inhibit
       tat-mediated or HIV-LTR transcription. The transcriptional inhibitory
       activity was not MHC class I restricted and could be mediated by a
       soluble factor(s). Supernatants from some CD8+ T lymphocyte cultures
       from HIV-1+ individuals exerted an inhibitory effect on tat-mediated
       HIV-LTR transcription comparable to that seen with CD8+ cells. In
       conclusion, CD8+ lymphocytes from asymptomatic HIV-1+ individuals could
       suppress virus production by inhibiting HIV-1 gene expression.(ABSTRACT
       TRUNCATED AT 250 WORDS)
 DE    Antigens, CD8  Cells, Cultured  Histocompatibility Antigens Class I
       Human  *HIV Long Terminal Repeat  HIV-1/*GENETICS/*IMMUNOLOGY/PHYSIOLOGY
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  T-Lymphocyte
       Subsets/*IMMUNOLOGY  Transcription, Genetic/IMMUNOLOGY  Virus
       Replication/GENETICS/IMMUNOLOGY  JOURNAL ARTICLE

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