Document 0359
 DOCN  M9440359
 TI    Accumulation and metabolism of adenallene by murine leukemia L1210
       cells.
 DT    9404
 AU    Kessel D; Zemlicka J; Department of Pharmacology and Medicine, Wayne
       State University,; Detroit, Michigan 48201.
 SO    Arch Biochem Biophys. 1994 Jan;308(1):222-5. Unique Identifier :
       AIDSLINE MED/94145083
 AB    Accumulation of the anti-HIV agent adenallene was examined in murine
       leukemia L1210 cells. Initial studies indicated an unusual result:
       accumulation of labeled adenallene was enhanced by decreasing the
       temperature of incubation, and (at 37 degrees C) by extracellular
       adenine, but not adenosine, cytosine, or thymine. We found that these
       phenomena resulted from the rapid deamination of intracellular
       adenallene to hypoxallene. Exodus of the latter was impaired at low
       temperatures and antagonized by hypoxanthine, the deamination product of
       adenine. In the presence of a deaminase inhibitor, adenallene transport
       was temperature-insensitive and nonsaturable and not affected by
       nucleoside transport inhibitors (dilazep, nitrobenzylthioinosine, and
       dipyridamole). These results support the view that adenallene enters
       cells by diffusion and is deaminated to hypoxallene, whose exodus occurs
       via a temperature-sensitive process exhibiting some structural
       specificity. We found no evidence of adenallene phosphorylation in L1210
       cells.
 DE    Adenine/*ANALOGS & DERIVATIVES/METABOLISM/PHARMACOLOGY
       Adenosine/PHARMACOLOGY  Animal  Antiviral Agents/*METABOLISM  Biological
       Transport/DRUG EFFECTS  Biotransformation  Cytosine/PHARMACOLOGY
       Kinetics  Leukemia L1210/*METABOLISM  Mice  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  Temperature  Thymine/PHARMACOLOGY  Tritium
       Tumor Cells, Cultured  JOURNAL ARTICLE

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