Document 0439
 DOCN  M9440439
 TI    Comparison of a lysed whole blood method to purified cell preparations
       for lymphocyte immunophenotyping: differences between healthy controls
       and HIV-positive specimens.
 DT    9404
 AU    Tamul KR; O'Gorman MR; Donovan M; Schmitz JL; Folds JD; Clinical
       Microbiology-Immunology Laboratory, University of North; Carolina
       Hospitals, Chapel Hill 27514.
 SO    J Immunol Methods. 1994 Jan 3;167(1-2):237-43. Unique Identifier :
       AIDSLINE MED/94141199
 AB    Although the majority of clinical laboratories now use a lysed whole
       blood (LWB) method for routine immunophenotyping, researchers wishing to
       perform other types of studies with lymphocytes from HIV+ patients may
       still need to use purified cell preparations, such as peripheral blood
       mononuclear cells (PBMC). A comparison study of the two methods was
       performed, using peripheral blood specimens from normal donors and from
       patients infected with the human immunodeficiency virus (HIV+).
       Reproducibility studies and several types of holding studies (both
       before and after specimen processing) were also performed. The results
       suggest that the two different methods of sample preparation have
       different effects upon abnormal patient specimens than those observed in
       healthy controls. Immunophenotyping results derived from the two
       different methods cannot be considered equivalent for the purposes of
       quantitating the presence of a particular type of cell.
 DE    Antigens, CD/*ANALYSIS  Flow Cytometry  Hemolysis  Human  HIV
       Seropositivity/*BLOOD  Immunophenotyping/*METHODS  Infant, Newborn
       Specimen Handling  T-Lymphocyte Subsets/*IMMUNOLOGY  JOURNAL ARTICLE

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       protected by Copyright Law (Title 17, U.S.Code).