Document 0443
 DOCN  M9440443
 TI    Virological diagnosis of progressive multifocal leukoencephalopathy:
       detection of JC virus DNA in cerebrospinal fluid and brain tissue of
       AIDS patients.
 DT    9404
 AU    Moret H; Guichard M; Matheron S; Katlama C; Sazdovitch V; Huraux JM;
       Ingrand D; Laboratoire de Microbiologie, Hopital Robert Debre, Reims,;
       France.
 SO    J Clin Microbiol. 1993 Dec;31(12):3310-3. Unique Identifier : AIDSLINE
       MED/94141009
 AB    Cerebrospinal fluid (CSF) from 12 AIDS patients with clinical signs
       consistent with progressive multifocal leukoencephalopathy (PML) was
       examined by the polymerase chain reaction (PCR) for the presence of JC
       virus (JCV). A specific JCV target sequence was amplified in the CSF
       from 9 of the 12 patients and also in brain tissue from all nine
       JCV-positive patients. The clinical course, neuroimaging features, and,
       in four cases, histopathology of brain tissue proved that the nine
       patients had PML. In the other three patients with central nervous
       system disorders, the JCV genome was undetectable by PCR and Southern
       blot analysis in CSF and brain tissue. The clinical course and
       neuroimaging features ruled out PML in these three patients. Five CSF
       samples and five brain tissue specimens from 10 PML-free AIDS patients
       with central nervous system disorders were all negative for the JCV
       genome by PCR and Southern blot analysis. These results show that
       detection of JCV in CSF by PCR is a good alternative to brain tissue
       studies for the virological diagnosis of PML in AIDS patients.
 DE    AIDS-Related Opportunistic Infections/*DIAGNOSIS/MICROBIOLOGY  Blotting,
       Southern  Brain/MICROBIOLOGY  DNA, Viral/CEREBROSPINAL FLUID/ISOLATION &
       PURIF  Human  Leukoencephalopathy, Progressive Multifocal/COMPLICATIONS/
       *DIAGNOSIS/MICROBIOLOGY  Papovaviridae
       Infections/COMPLICATIONS/*DIAGNOSIS/MICROBIOLOGY  Polymerase Chain
       Reaction  Polyomavirus hominis 2/*GENETICS/*ISOLATION & PURIF  Support,
       Non-U.S. Gov't  Tumor Virus
       Infections/COMPLICATIONS/*DIAGNOSIS/MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).