Document 0444
 DOCN  M9440444
 TI    Karyotyping of Cryptococcus neoformans as an epidemiological tool.
 DT    9404
 AU    Perfect JR; Ketabchi N; Cox GM; Ingram CW; Beiser CL; Department of
       Medicine, Duke University Medical Center, Durham,; North Carolina 27710.
 SO    J Clin Microbiol. 1993 Dec;31(12):3305-9. Unique Identifier : AIDSLINE
       MED/94141008
 AB    Karyotyping of Cryptococcus neoformans var. neoformans can be used as an
       epidemiological tool for C. neoformans infections. In this study of over
       40 isolates from both clinical and environmental sources, 90% had a
       unique chromosome banding by pulsed-field electrophoresis. There was no
       conserved pattern associated with body site of infection, geographical
       location of the isolate, or human immunodeficiency virus status.
       Karyotypes of individual isolates remained stable during both in vitro
       passage and in vivo infections. Karyotype was used to exclude the
       possibility of nosocomial spread of C. neoformans in one clinical
       situation and supported relapse in two other cases. Because of its
       variable sizes between isolates, karyotyping of C. neoformans is a
       convenient method for molecular identification of different strains.
 DE    Adult  Animal  AIDS-Related Opportunistic Infections/MICROBIOLOGY  Case
       Report  Chromosomes, Fungal  Cross Infection/MICROBIOLOGY
       Cryptococcosis/COMPLICATIONS/EPIDEMIOLOGY/*MICROBIOLOGY  Cryptococcus
       neoformans/*GENETICS/ISOLATION & PURIF  Disease Models, Animal  DNA,
       Fungal/ISOLATION & PURIF  Environmental Microbiology  Epidemiologic
       Methods  Human  Karyotyping  Male  Meningitis,
       Cryptococcal/EPIDEMIOLOGY/MICROBIOLOGY  Rabbits  Recurrence  Support,
       U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).