Document 0445
 DOCN  M9440445
 TI    A new trichrome-blue stain for detection of microsporidial species in
       urine, stool, and nasopharyngeal specimens.
 DT    9404
 AU    Ryan NJ; Sutherland G; Coughlan K; Globan M; Doultree J; Marshall J;
       Baird RW; Pedersen J; Dwyer B; Victorian Infectious Diseases Reference
       Laboratory, Fairfield; Hospital, Victoria, Australia.
 SO    J Clin Microbiol. 1993 Dec;31(12):3264-9. Unique Identifier : AIDSLINE
       MED/94141002
 AB    Detection of microsporidia in clinical specimens has relied on electron
       microscopy, histology, or staining. This article describes further
       alterations to the modified trichrome staining method which make it
       easier to identify microsporidial spores. The changes are a decrease in
       the phosphotungstic acid level and the substitution of a colorfast
       counterstain, aniline blue, for the fast green of the original stain.
       The modified stain provides good contrast between microsporidial spores
       and background material including human and fungal cells. Stool
       specimens from 139 human immunodeficiency virus-seropositive patients
       revealed that 5 patients were infected with Enterocytozoon bieneusi and
       6 patients had larger spores. Thin-section electron microscopy of the
       larger spores showed a structure consistent with that of either
       Encephalitozoon or Septata species. Three of the patients with
       Encephalitozoon- or Septata-like species had disseminated infection,
       with spores detected in nasopharyngeal aspirates and urine samples.
 DE    Animal  *Azo Compounds  AIDS-Related Opportunistic
       Infections/PARASITOLOGY  Encephalitozoon/ISOLATION &
       PURIF/ULTRASTRUCTURE  *Eosine Yellowish-(YS)  Evaluation Studies
       Feces/PARASITOLOGY  Fluorescent Dyes  Human  *Methyl Green  Microscopy,
       Electron  Microspora Infections/COMPLICATIONS/PARASITOLOGY
       Microsporida/*ISOLATION & PURIF/ULTRASTRUCTURE  Nasopharynx/PARASITOLOGY
       Parasitology/*METHODS  Spores/ISOLATION & PURIF/ULTRASTRUCTURE  Stains
       and Staining/*METHODS  Urine/PARASITOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).