Document 0448
 DOCN  M9440448
 TI    HIV-induced syncytia of a T cell line form single giant pseudopods and
       are motile.
 DT    9404
 AU    Sylwester A; Wessels D; Anderson SA; Warren RQ; Shutt DC; Kennedy RC;
       Soll DR; Department of Biological Sciences, University of Iowa, Iowa
       City; 52242.
 SO    J Cell Sci. 1993 Nov;106 ( Pt 3):941-53. Unique Identifier : AIDSLINE
       MED/94140946
 AB    The human immunodeficiency virus, HIV, induces syncytium formation in
       cultures of many T cell lines. These syncytia have previously been
       viewed as disorganized fusion products in the throes of death. Evidence
       is presented that in HIV1-infected SupT1 cultures, syncytia five times
       to over one hundred times larger than single cells organize their many
       nuclei into blastula-like balls, reorganize their cytoskeleton to mimic
       that of a single cell, and extend single, giant pseudopods in a polar
       fashion. Medium-sized syncytia are capable of translocation through
       extension of these giant pseudopods. The rate of translocation of
       syncytia is comparable to that of single cells. Single cell motility,
       syncytium motility and pseudopod extension also appear to play roles in
       the recruitment of cells into syncytia. Finally, condensation of F-actin
       at cell-syncytium and syncytium-syncytium adhesion sites suggests the
       involvement of the cytoskeleton in the adhesion and/or subsequent fusion
       event. These results suggest that the fusion events involved in
       HIV-induced syncytia formation involve both cell motility and
       reorganization of the cytoskeleton, and demonstrate that syncytia are
       highly organized, motile entities.
 DE    Actins/METABOLISM  Cell Adhesion  Cell Line  Cell Movement
       Cytoskeleton/ULTRASTRUCTURE  Giant Cells/*PHYSIOLOGY/*ULTRASTRUCTURE
       Human  HIV-1/*PHYSIOLOGY  Microscopy, Electron, Scanning
       Pseudopodia/*ULTRASTRUCTURE  Support, U.S. Gov't, Non-P.H.S.  Support,
       U.S. Gov't, P.H.S.
       T-Lymphocytes/*MICROBIOLOGY/PHYSIOLOGY/ULTRASTRUCTURE  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).