Document 0451
 DOCN  M9440451
 TI    Specificity of priming reaction of HIV-1 reverse transcriptase, 2'-OH or
       3'-OH.
 DT    9404
 AU    Shimada M; Hosaka H; Takaku H; Smith JS; Roth MJ; Inouye S; Inouye M;
       Department of Biochemistry, Robert Wood Johnson Medical School,;
       Piscataway, New Jersey 08854.
 SO    J Biol Chem. 1994 Feb 11;269(6):3925-7. Unique Identifier : AIDSLINE
       MED/94140799
 AB    It has not been unambiguously demonstrated whether the priming reaction
       of human immunodeficiency virus, type 1 (HIV-1) cDNA synthesis initiates
       with either the 2'-OH or 3'-OH group of the 3'-terminal adenosine
       residue of tRNA(Lys-3). In this report, we synthesized tRNA(Lys-3) of
       which the 3'-terminal adenosine residue lacks either a 2'-OH or 3'-OH.
       These tRNA molecules were used for the HIV-1 cDNA-priming reaction in a
       cell-free system consisting of a 141-base RNA template and purified
       HIV-1 reverse transcriptase. It was found that under the conditions
       used, the tRNA containing the 2'-deoxyadenosine was able to initiate the
       cDNA synthesis, while the tRNA with the 3'-deoxyadenosine was not. The
       results show that retroviral reverse transcriptase specifically primes
       cDNA synthesis from the 3'-OH group. This is in contrast to bacterial
       reverse transcriptase, which initiates cDNA synthesis from the 2'-OH
       group of an internal guanosine residue of a template RNA.
 DE    Base Sequence  Cell-Free System  DNA Primers/CHEMISTRY  DNA,
       Complementary/BIOSYNTHESIS  HIV-1/ENZYMOLOGY  Molecular Sequence Data
       Reverse Transcriptase/*METABOLISM  RNA, Transfer, Lys/METABOLISM
       Substrate Specificity  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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