Document 0471
 DOCN  M9440471
 TI    Effects of calcium ions on proteolytic processing of HIV-1 gp160
       precursor and on cell fusion.
 DT    9404
 AU    Moulard M; Montagnier L; Bahraoui E; Laboratorie de Biochimie, CNRS URA
       1455-Ingenierie des; Proteines, Faculte de Medecine Secteur Nord,
       Marseille,; France.
 SO    FEBS Lett. 1994 Feb 7;338(3):281-4. Unique Identifier : AIDSLINE
       MED/94139940
 AB    Complete activation of human immunodeficiency virus type 1 (HIV-1)
       requires the endoproteolytic cleavage by cellular protease of the
       envelope glycoprotein precursor (gp160) into the external glycoprotein
       gp120, and the transmembrane glycoprotein gp41. We report here the
       effect of depletion of cellular calcium ions on maturation of precursor
       gp160 and its concomitant effect on syncytium formation. We show that
       the cellular endoprotease activity responsible for gp160 maturation and
       the capacity for HIV-1 to induce syncytium formation are
       calcium-dependent. In addition, we show that endoproteolytic maturation
       is a key step in syncytium formation induced by HIV-1.
 DE    Amino Acid Sequence  Calcium/*PHARMACOLOGY  Cell Fusion  Cell Line  Gene
       Products, env/*METABOLISM  *Giant Cells  HIV-1/*METABOLISM  Molecular
       Sequence Data  Protein Precursors/*METABOLISM  *Protein Processing,
       Post-Translational  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).