Document 0479
 DOCN  M9440479
 TI    In vitro manipulation of early mouse embryos induces HIV1-LTRlacZ
       transgene expression.
 DT    9404
 AU    Vernet M; Cavard C; Zider A; Fergelot P; Grimber G; Briand P; Institut
       Cochin de Genetique Moleculaire, Laboratoire de; Genetique et Pathologie
       Experimentales-CJF INSERM 90-03,; Paris, France.
 SO    Development. 1993 Dec;119(4):1293-300. Unique Identifier : AIDSLINE
       MED/94139569
 AB    We report here that the transcriptional activity of early mouse embryos
       is affected by their manipulation and culture in vitro, using transgenic
       embryos that express the reporter gene lacZ. We examined the pattern of
       expression of the lacZ gene fused to the human immunodeficiency virus
       type 1 long terminal repeat during the preimplantation stages. Transgene
       expression is induced as early as the two-cell stage in embryos
       developed in vitro, while there is no constitutive expression at the
       same stage in embryos developed in vivo. We have established a relation
       between this inducible expression occurring in vitro and an oxidative
       stress phenomenon. Indeed, when the culture medium is supplemented with
       antioxidants such N-acetyl-cysteine or CuZn-superoxide dismutase the
       transgene expression is markedly reduced. We also present evidence that
       the transgene expression in vitro coincides with the onset of the
       embryonic genome activation as attested by the synthesis of the 70 x
       10(3) M(r) protein complex. Therefore, this transgene expression could
       prove to be a useful tool in our understanding of the molecular
       mechanisms involved in this crucial developmental event.
 DE    Animal  Autoradiography  Cells, Cultured  Electrophoresis,
       Polyacrylamide Gel  Gene Expression Regulation/*PHYSIOLOGY  Genes,
       Viral/*GENETICS  HIV-1/*GENETICS  Lac Operon/*GENETICS  Mice  Mice,
       Transgenic/EMBRYOLOGY/GENETICS  Oxidation-Reduction  Support, Non-U.S.
       Gov't  Transcription, Genetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).