Document 0492
 DOCN  M9440492
 TI    Use of the cytomegalovirus antigenemia (CMV-Ag) assay for the detection
       of CMV in the blood of AIDS patients.
 DT    9404
 AU    Lipson SM; Kaplan MH; Tseng LF; Mandel FS; Jane and Dayton Brown and
       Dayton T. Brown, Jr., Virology; Laboratory, Department of Medicine,
       North Shore University; Hospital-Cornell University Medical College,
       Manhasset, NY 11030.
 SO    Can J Microbiol. 1993 Nov;39(11):1059-65. Unique Identifier : AIDSLINE
       MED/94138794
 AB    Direct specimen testing was performed on 186 peripheral blood specimens
       to identify the presence of antigen to cytomegalovirus (viz., the
       cytomegalovirus antigenemia (CMV-Ag) assay). Confirmatory testing was
       performed using the shell vial indirect immunofluorescence assay
       (SVA-IFA), the indirect immunoperoxidase assay (TC-IPA), and
       conventional tube culture isolation (TC-CPE). The primary reagent for
       the CMV-Ag assay consisted of anti-CMV monoclonal antibody directed
       against the internal matrix structural phosphoprotein (1C3;
       Clonatec-Biosoft, France). The 72-kDa early nuclear antigen (Dupont) was
       utilized in the SVA-IFA and the TC-IPA. All test systems received an
       equal number of polymorphonuclear leukocytes in the inoculum. CMV was
       detected and isolated from 30% (55/186) of the specimens evaluated by
       either one or a combination of the tests. Detection and (or) isolation
       of CMV from blood by the CMV-Ag assay, SV-IFA, TC-IPA, and TC-CPE
       occurred at a rate of 17 (31/186), 12 (22/186), 16 (29/186), and 26%
       (49/186). Three of 55 positive specimens were identified only by the
       CMV-Ag assay; each patient in question, however, had at least one
       previous CMV isolate. No significant differences in sensitivity occurred
       between the CMV-Ag assay, the SVA-IFA, or the TC-IPA. However, TC-CPE
       including the blind passage of all negative tube cultures yielded a
       significantly larger number of positive blood specimens than either of
       the rapid detection methodologies. The CMV-Ag assay encompasses the
       benefits of a nonculture system, is simple to perform and easy to read,
       permits a same-day diagnosis, and requires less reagents than the
       routinely used SVA-IFA or TC-IPA.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Acquired Immunodeficiency Syndrome/*COMPLICATIONS  Antigens,
       Viral/*BLOOD  Blood/MICROBIOLOGY  Comparative Study
       Cytomegalovirus/*ISOLATION & PURIF  Cytomegalovirus
       Infections/COMPLICATIONS/*DIAGNOSIS/IMMUNOLOGY  Human  Immunocompromised
       Host  Serodiagnosis/*METHODS  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).