Document 0495
 DOCN  M9440495
 TI    Abnormal lipid composition of bronchoalveolar lavage fluid obtained from
       individuals with AIDS-related lung disease.
 DT    9404
 AU    Rose RM; Catalano PJ; Koziel H; Furlong ST; Division of Pulmonary and
       Critical Care Medicine, New England; Deaconess Hospital, Boston,
       Massachusetts.
 SO    Am J Respir Crit Care Med. 1994 Feb;149(2 Pt 1):332-8. Unique Identifier
       : AIDSLINE MED/94138591
 AB    Surfactant lipids are not only important to the physiologic function of
       the lungs, but may also influence disease processes like Pneumocystis
       pneumonia, in which the interaction of host-defense cells with pathogen
       occurs within the confines of the surfactant-rich alveolar hypophase. In
       the present studies the lipid profile of bronchoalveolar lavage fluid
       (BALF) was characterized in subjects with AIDS-related lung diseases
       including Pneumocystis pneumonia. BALF lipid and total protein
       measurements were made in 43 subjects with acquired immune deficiency
       syndrome (AIDS)-related lung disease and compared with those made in 50
       normal human immunodeficiency virus (HIV)-seronegative controls. The
       AIDS patient samples contained significantly greater amounts of total
       cholesterol, phosphatidylglycerol (PG), and protein than the control
       samples; in contrast to previous observations in rodent P. carinii
       infection, no differences were seen in total phospholipid (PL) or
       phosphatidylcholine (PC) in the two groups. The proportions of several
       of these lipids were deranged in BALF obtained from the patient group:
       PG/PL and PC/cholesterol differed significantly from normal samples. In
       the subset of patients with AIDS-related Pneumocystis pneumonia, no
       correlation was apparent between discrete BALF lipids and clinical
       indices reflective of disease severity. Using these measurements to
       approximate the lipid composition of the alveolar microenvironment in
       AIDS-related lung disease, we performed experiments in which normal
       human alveolar macrophages were exposed to exogenous liposomal lipids
       and then challenged with P. carinii. The ingestion but not binding of P.
       carinii by macrophages was diminished as a result of lipid
       exposure.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Adult  AIDS-Related Opportunistic Infections/*METABOLISM
       Bronchoalveolar Lavage Fluid/*CHEMISTRY  Enzyme-Linked Immunosorbent
       Assay  Female  Human  Lipids/*ANALYSIS  Macrophages, Alveolar/IMMUNOLOGY
       Male  Pneumocystis carinii/IMMUNOLOGY  Pneumonia, Pneumocystis
       carinii/*METABOLISM  Pulmonary Surfactants/*ANALYSIS  Support, U.S.
       Gov't, P.H.S.  JOURNAL ARTICLE

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