Document 0542
 DOCN  M9440542
 TI    Human immunodeficiency virus infection of early passage cervical
       epithelial cultures.
 DT    9404
 AU    Patrick SL; Wright TC; Fox HE; Ginsberg HS; Department of Obstetrics and
       Gynecology, Columbia University,; Columbia Presbyterian Medical Center,
       New York, NY 10032.
 SO    Int J STD AIDS. 1993 Nov-Dec;4(6):342-5. Unique Identifier : AIDSLINE
       MED/94137870
 AB    Women are infected with HIV in increasing numbers; the predominant mode
       of spread is through heterosexual transmission. Little is known
       regarding the mechanism of HIV transit through the female genital tract.
       We investigated whether early passage cervical epithelial cells could be
       directly infected with HIV-1LAI. Virus production was measured using the
       reverse transcriptase (RT) assay and direct assay for syncytia-forming
       units. In-situ hybridization was performed on infected cervical cell
       cultures. Immunostaining was carried out using a monoclonal antibody to
       leukocyte common antigen (LCA). Virus was recovered in the supernatants
       of all infected cervical cultures. Localization of HIV infection using
       in-situ hybridization identified rare cells in the population which gave
       a strong signal. These infected cells had a lymphoid morphology and were
       also detected using immunostaining for LAC. Cervical epithelial cells
       were uninfected in this in vitro model; cells in this population which
       supported viral replication were most likely of the macrophage/monocyte
       lineage.
 DE    Cells, Cultured  Cervix Uteri/*MICROBIOLOGY/PATHOLOGY
       Epithelium/MICROBIOLOGY/PATHOLOGY  Female  Human  HIV
       Infections/PATHOLOGY/*TRANSMISSION  HIV-1/*PATHOGENICITY  Models,
       Biological  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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