Document 0592
 DOCN  M9440592
 TI    Presence of papova-like viral particles in cerebrospinal fluid of AIDS
       patients with progressive multifocal leukoencephalopathy. An additional
       test for in vivo diagnosis.
 DT    9404
 AU    Orefice G; Campanella G; Cicciarello S; Chirianni A; Borgia G; Rubino S;
       Mainolfi M; Coppola M; Piazza M; Second Department of Neurology, Medical
       School, Federico II; University, Naples, Italy.
 SO    Acta Neurol (Napoli). 1993 Oct;15(5):328-32. Unique Identifier :
       AIDSLINE MED/94136187
 AB    An in vivo diagnosis of progressive multifocal leukoencephalopathy
       (PML), a neurological opportunistic viral infection in AIDS patients,
       can be made only by brain biopsy. In order to identify viral particles,
       we examined the cerebrospinal fluid (CSF) of 15 AIDS patients with focal
       neurological signs by electron microscopy using negative staining
       technique. In 2 out of 3 patients with clinical and neuroradiological
       presumptive diagnosis of PML, the CSF examination revealed papova-like
       viral particles. Our results support the hypothesis that the severe
       cell-mediated immunodeficiency reactivates papovavirus from a latent
       state in the brain, leading to PML. Therefore, the CSF study by negative
       staining might be a useful test for an in vivo diagnosis of PML.
 DE    Acquired Immunodeficiency Syndrome/*CEREBROSPINAL FLUID/  COMPLICATIONS
       Brain/MICROBIOLOGY/RADIOGRAPHY  Cerebrospinal Fluid/*MICROBIOLOGY
       Comorbidity  Female  Human  Immunosuppression  Leukoencephalopathy,
       Progressive Multifocal/COMPLICATIONS/  *DIAGNOSIS/MICROBIOLOGY  Male
       Microscopy, Electron  Papovaviridae/*ISOLATION & PURIF  Tomography,
       X-Ray Computed  JOURNAL ARTICLE

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