Document 0595
 DOCN  M9440595
 TI    [The isolation and characteristics of monoclonal antibodies to
       recombinant proteins of HIV-1 and HIV-2--the env and gag gene products]
 DT    9404
 AU    Pugach AV; Zverev VV; Pille ER; Shukhmina NR; Mel'nikova NL; Nosik DN;
       Maliushova VV; Andzhaparidze OG
 SO    Vopr Virusol. 1993 Nov-Dec;38(6):253-5. Unique Identifier : AIDSLINE
       MED/94135954
 AB    Ten hybridomas secreting monoclonal antibodies (Mab) against recombinant
       HIV-1 and HIV-2 antigens were produced (3 Mab anti-gag protein, 2
       anti-env1, and 5 anti-env2). In the immunoblotting assay all the
       anti-gag Mabs reacted with HIV capsid protein p24, whereas one of them
       reacted also with p55 protein and with 7 other polypeptides. Another
       anti-gag Mab cross-reacted with the antigen of subpopulation of human
       peripheral blood lymphocytes. The third one interacted with the antigen
       of both HIV-1 and HIV-2. All the 10 Mabs interacted with natural HIV
       antigens and can be used for identification and differentiation of HIV-1
       and HIV-2.
 DE    Animal  Antibodies, Monoclonal/ANALYSIS/*ISOLATION & PURIF  Cross
       Reactions  English Abstract  Fluorescent Antibody Technique  Gene
       Products, env/*IMMUNOLOGY  Gene Products, gag/*IMMUNOLOGY  Human
       Hybridomas/IMMUNOLOGY  HIV Antigens/BLOOD  HIV-1/*IMMUNOLOGY
       HIV-2/*IMMUNOLOGY  Immunization/METHODS  Immunoblotting  Mice  Mice,
       Inbred BALB C  Recombinant Proteins/IMMUNOLOGY  JOURNAL ARTICLE

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