Document 0599
 DOCN  M9440599
 TI    MHC-I non-restricted cytotoxic activity in Macaca sylvana experimentally
       inoculated with HIV2 and SIV/mac.
 DT    9404
 AU    Charaf B; Sanhadji K; Sekkat S; Farouqui B; Touraine JL; Benslimane A;
       Laboratoire d'Immunovirologie, Institut Pasteur du Maroc,; Casablanca.
 SO    Thymus. 1993 Aug;22(1):1-12. Unique Identifier : AIDSLINE MED/94135814
 AB    The anti-retrovirus cell-mediated immunity was repeatedly investigated
       in seven monkeys (Macaca sylvana). Four of these animals were injected
       with cell-free supernatants containing human immunodeficiency viruses:
       two monkeys received HIV1 Bru (2.5 x 10(6) cpm), two received HIV2 Rod
       (1.5 x 10(6) cpm). Two additional animals were injected with a cell-free
       supernatant containing simian immunodeficiency virus SIV/mac 251 (1.5 x
       10(6) cpm) and the last animal served as control. The four macaques
       infected with HIV2 Rod and SIV/mac 251 seroconverted. Freshly isolated
       and non stimulated peripheral blood mononuclear cells from these
       infected macaques and from the uninfected control were repeatedly
       assessed for cytolytic activity. Target cells consisted of heterologous
       human cell lines expressing HIV1 Bru, HIV2 Rod or SIV/mac proteins. A
       significant cytotoxic activity, non-restricted at the major
       histocompatibility complex class I (MHC-I), was demonstrated in one HIV2
       Rod-infected animal (F8) and in one SIV/mac 251-infected animal (M1).
       This last animal showed progressively diminishing cytolytic activity
       that was correlated with a pronounced decrease in CD4+ lymphocytes. An
       AIDS-like disease developed in M1, with presence of lymphadenopathy,
       weight loss, diarrhea and opportunistic infections. Cytotoxic activity
       was active against SIV and HIV2-infected target cells in an
       MHC-unrestricted manner; it was specific to virus-infected cells and
       there was cross-reactivity between HIV2 and SIV. Cytotoxic effectors
       appeared to be mainly CD8+ cells. This model may prove to be very useful
       in evaluating the capacity of candidate AIDS vaccines to elicit
       effective cell-mediated immune responses.
 DE    Acquired Immunodeficiency Syndrome/IMMUNOLOGY  Animal  Cell Line
       Comparative Study  *Cytotoxicity, Immunologic  *Disease Models, Animal
       Human  HIV Infections/*IMMUNOLOGY  *HIV-1/PATHOGENICITY
       *HIV-2/PATHOGENICITY  Immunity, Cellular  Leukocyte Count
       Macaca/*IMMUNOLOGY  Simian Acquired Immunodeficiency
       Syndrome/*IMMUNOLOGY  *SIV/PATHOGENICITY  T-Lymphocytes,
       Cytotoxic/IMMUNOLOGY  T4 Lymphocytes  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).