Document 0601
 DOCN  M9440601
 TI    Relation of HIV-I in bronchoalveolar lavage cells to abnormalities of
       lung function and to the presence of Pneumocystis pneumonia in HIV-I
       seropositive patients.
 DT    9404
 AU    Clarke JR; Taylor IK; Fleming J; Williamson JD; Mitchell DM; Department
       of Medical Microbiology, St Mary's Hospital Medical; School, London.
 SO    Thorax. 1993 Dec;48(12):1222-6. Unique Identifier : AIDSLINE
       MED/94135643
 AB    BACKGROUND--HIV is present in bronchoalveolar lavage cells of some but
       not all HIV seropositive patients. Abnormalities of lung function have
       been described in such patients in the absence of clinically overt
       pneumonia or other respiratory infections. It is possible that the
       presence of HIV in alveolar macrophages could account for these
       abnormalities. It is also possible that the presence of HIV in alveolar
       macrophages contributes to immunosuppression and an increased incidence
       of opportunistic infections. METHODS--This was a prospective study of
       157 HIV seropositive patients requiring diagnostic bronchoscopy for
       investigation of new respiratory symptoms, chest radiograph abnormality,
       or pneumonic illness. Presence of HIV in bronchoalveolar lavage cells
       obtained at diagnostic bronchoscopy was determined by polymerase chain
       reaction to detect proviral DNA and in vitro cocultivation to detect
       productive virus infection. With these two techniques the presence or
       absence of HIV in bronchoalveolar lavage was compared with the presence
       of abnormalities of lung function or presence of Pneumocystis pneumonia.
       RESULTS--HIV was detected in bronchoalveolar lavage cells in 65% of
       patients by means of the polymerase chain reaction and 59% with
       cocultivation. With both methods of detection there was no association
       between the presence or absence of HIV and the presence of Pneumocystis
       pneumonia; nor was there a relation between the presence of HIV and
       abnormalities of lung function. CONCLUSION--The presence of HIV in
       bronchoalveolar lavage cells does not predispose to an increased
       incidence of Pneumocystis pneumonia; nor does it contribute to
       abnormalities of lung function.
 DE    Antigens, CD4/ANALYSIS  Bronchoalveolar Lavage
       Fluid/CYTOLOGY/*MICROBIOLOGY  DNA, Viral/ANALYSIS  Female  HIV
       Seropositivity/COMPLICATIONS/*MICROBIOLOGY  HIV-1/GENETICS/*ISOLATION &
       PURIF  Human  Lung Diseases/*ETIOLOGY/MICROBIOLOGY  Male  Pneumonia,
       Pneumocystis carinii/*ETIOLOGY/MICROBIOLOGY  Polymerase Chain Reaction
       Prospective Studies  Proviruses/GENETICS  Support, Non-U.S. Gov't
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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