Document 0681
 DOCN  M9440681
 TI    Association of HTLV-I antibody profile of asymptomatic carriers with
       proviral DNA levels of peripheral blood mononuclear cells.
 DT    9404
 AU    Ishihara S; Okayama A; Stuver S; Horinouchi H; Shioiri S; Murai K;
       Kubota T; Yamashita R; Tachibana N; Tsubouchi H; et al; Second
       Department of Medicine, Miyazaki Medical College, Japan.
 SO    J Acquir Immune Defic Syndr. 1994 Feb;7(2):199-203. Unique Identifier :
       AIDSLINE MED/94133093
 AB    The human T-lymphotropic virus type I (HTLV-I) antibody profile of 216
       asymptomatic carriers in Miyazaki, Japan, was analyzed in conjunction
       with the HTLV-I proviral DNA levels in their peripheral blood
       mononuclear cells (PBMC) determined by the semiquantitative polymerase
       chain reaction (PCR) method. The geometric mean HTLV-I titer by particle
       agglutination assay (PA) of 58 subjects (27%) with a high DNA level was
       1:1, 240; 94 (44%) with a medium DNA level, 1:740; 38 (18%) with a low
       level, 1:476; and, 26 (12%) with an undetectable level, 1:263. Moreover,
       when the subjects were divided into four groups according to titer from
       high to low, the correlation between DNA level and antibody titer level
       was highly significant (p < 0.0001). HTLV-I antibody subclass by Western
       blot (WB) was determined for 78 randomly selected samples from these
       carriers. Immunoglobulin (Ig) M antibody was detected in 35 (45%). The
       mean PA antibody titer was higher in carriers with IgM antibody than in
       those without, at each detectable proviral DNA level. These findings
       suggest that HTLV-I antibody titer is related to proviral DNA level and
       also to the presence of IgM antibodies among those with proviral DNA of
       the same level. Seven carriers (9%) were negative for IgG antibody by
       WB, among whom the proviral DNA level was low or undetectable and the PA
       titer was also low. Three of these were positive only for IgM antibody.
 DE    Aged  Agglutination Tests  Blotting, Western  Carrier State/*BLOOD
       Cohort Studies  DNA, Viral/BLOOD  Female  Human  HTLV-I/*GENETICS
       HTLV-I Antibodies/*BLOOD  HTLV-I Infections/*BLOOD  IgM/BLOOD
       Leukocytes, Mononuclear/*MICROBIOLOGY  Male  Middle Age  Polymerase
       Chain Reaction  Proviruses/GENETICS  JOURNAL ARTICLE

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