Document 0695
 DOCN  M9440695
 TI    Neutralization epitopes on HIV pseudotyped with HTLV-I: conservation of
       carbohydrate epitopes.
 DT    9404
 AU    Sorensen AM; Nielsen C; Arendrup M; Clausen H; Nielsen JO; Osinaga E;
       Roseto A; Hansen JE; Department of Infectious Diseases, University of
       Copenhagen,; Hvidovre Hospital, Denmark.
 SO    J Acquir Immune Defic Syndr. 1994 Feb;7(2):116-23. Unique Identifier :
       AIDSLINE MED/94133079
 AB    One mechanism for expanding the cellular tropism of human
       immunodeficiency virus (HIV) in vitro is through formation of
       phenotypically mixed particles (pseudotypes) with human T lymphotropic
       virus type I (HTLV-I). In this study we found that pseudotypes allow
       penetration of HIV particles into CD4-negative cells, previously
       nonsusceptible to HIV infection. The infection of CD4-negative cells
       with pseudotypes could be blocked with anti-HTLV-I serum but failed to
       be significantly inhibited with anti-HIV serum or a V3-neutralizing
       anti-gp120 monoclonal antibody. This may represent a possibility for
       pseudotypes to escape neutralization by the immune system in vivo.
       Previous reports have suggested that carbohydrate structures may be
       conserved neutralization epitopes on retroviruses. In this study, the
       neutralizing capacity of lectins and anti-carbohydrate monoclonal
       antibodies was found to block infection by cell-free pseudotypes in
       CD4-negative cells. We suggest that although viral cofactors might
       expand the tropism of HIV in vivo, HIV and HTLV-I seem to induce common
       carbohydrate neutralization epitopes.
 DE    Antibodies, Monoclonal/IMMUNOLOGY  Antigenic
       Determinants/ANALYSIS/IMMUNOLOGY  Carbohydrates/*IMMUNOLOGY  Cell Line
       Giant Cells/IMMUNOLOGY  Human  HIV/*IMMUNOLOGY/PHYSIOLOGY  HIV
       Antigens/ANALYSIS/*IMMUNOLOGY  HTLV-I/*IMMUNOLOGY  Immune
       Sera/IMMUNOLOGY  Lectins/IMMUNOLOGY  Neutralization Tests  Polymerase
       Chain Reaction  Proviruses/IMMUNOLOGY/PHYSIOLOGY
       Radioimmunoprecipitation Assay  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).