Document 0696
 DOCN  M9440696
 TI    Differential expression of tumor necrosis factor alpha and interleukin 1
       beta compared with interleukin 6 in monocytes from human
       immunodeficiency virus-positive individuals measured by polymerase chain
       reaction.
 DT    9404
 AU    Lathey JL; Kanangat S; Rouse BT; Department of Pediatrics, University of
       California, San Diego, La; Jolla 92093-0672.
 SO    J Acquir Immune Defic Syndr. 1994 Feb;7(2):109-15. Unique Identifier :
       AIDSLINE MED/94133078
 AB    Expression of tumor necrosis factor alpha (TNF alpha), interleukin 1
       beta (IL-1 beta), and interleukin 6 (IL-6) was evaluated in unstimulated
       peripheral blood monocytes obtained from human immunodeficiency
       virus-positive (HIV+) individuals using a reverse
       transcription-polymerase chain reaction (RT-PCR) method. In all, 40
       subjects were included--13 asymptomatic, 11 with ARC, seven with AIDS,
       and nine HIV- controls. Of the asymptomatic individuals, 85% were
       positive for TNF alpha and IL-1 beta compared with only 27% of the ARC
       and 42% of the AIDS patients. Expression of IL-6 message was observed in
       lesser proportions, with no significant differences among disease
       states. Quantitation of IL-1 beta and TNF alpha mRNA from the positive
       samples fell into two categories, low responders (six of 17), with <
       5,000 copies of IL-1 beta and TNF alpha mRNA, and high responders (11 of
       17), with > 5,000 copies per 10 pg of total cellular RNA. There was no
       correlation of mRNA detection or concentration with CD4+ cell number or
       beta 2-microglobulin levels. However, the levels of mRNA, but not its
       presence alone, were positively correlated with neopterin levels. The
       data show differential cytokine regulation in monocytes, observed as an
       increase in the expression of TNF alpha and IL-1 beta compared with IL-6
       in HIV+ patients. Our report also emphasizes the utility of an RT-PCR
       system in analyzing multiple cytokine transcript levels in small amounts
       of clinical materials.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/IMMUNOLOGY  AIDS-Related
       Complex/BLOOD/IMMUNOLOGY  Comparative Study  CD4-CD8 Ratio  *Gene
       Expression Regulation  Human  HIV Infections/BLOOD/*IMMUNOLOGY  HIV
       Seropositivity/BLOOD/IMMUNOLOGY  Interleukin-1/*BIOSYNTHESIS/GENETICS
       Interleukin-6/*BIOSYNTHESIS/GENETICS  Monocytes/*IMMUNOLOGY  Polymerase
       Chain Reaction  Regression Analysis  RNA, Messenger/ANALYSIS  Support,
       Non-U.S. Gov't  Transcription, Genetic  Tumor Necrosis
       Factor/*BIOSYNTHESIS/GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).