Document 0714
 DOCN  M9440714
 TI    Effects of CD45 on NF-kappa B. Implications for replication of HIV-1.
 DT    9404
 AU    Baur A; Garber S; Peterlin BM; Howard Hughes Medical Institute,
       University of California at San; Francisco 94143-0724.
 SO    J Immunol. 1994 Feb 1;152(3):976-83. Unique Identifier : AIDSLINE
       MED/94132650
 AB    Increased levels of replication of the HIV type 1 are observed after the
       activation of infected T cells through the TCR. However, anti-CD45
       antibodies inhibit these effects in cells from infected individuals. In
       this study, we examined interrelationships between CD45 and HIV-1
       further. We measured effects on the HIV-1 LTR in T cell lines that were
       stimulated with antibodies against CD45 and in those that lacked the
       expression of CD45 on their surfaces. First, anti-CD45 antibodies did
       not affect basal but decreased activated levels of expression from the
       HIV-1 LTR. Second, T cells, which lack CD45 and cannot signal via the
       TCR, supported higher levels of viral replication and gene expression.
       This was due to the presence of active NF-kappa B complexes in the
       nucleus of CD45- T cells. Additionally, infected T cells displayed lower
       levels of CD45 on their surfaces. Thus, CD45 plays an active role in the
       physiology of T cells and in the replication of HIV-1.
 DE    Antigens, CD45/*PHYSIOLOGY  Cell Nucleus/METABOLISM  *Gene Expression
       Regulation, Viral  Human  HIV/*GROWTH & DEVELOPMENT  HIV
       Infections/*IMMUNOLOGY  HIV Long Terminal Repeat  In Vitro  NF-kappa
       B/*PHYSIOLOGY  Receptors, Antigen, T-Cell/PHYSIOLOGY  RNA,
       Messenger/GENETICS  Support, Non-U.S. Gov't  Tumor Cells, Cultured  T4
       Lymphocytes/IMMUNOLOGY/*MICROBIOLOGY  Virus Replication  JOURNAL ARTICLE

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