Document 0716
 DOCN  M9440716
 TI    A monoclonal antibody that recognizes HLA-B27 in the context of
       peptides.
 DT    9404
 AU    Wang J; Yu DT; Fukazawa T; Kellner H; Wen J; Cheng XK; Roth G; Williams
       KM; Raybourne RB; Department of Medicine, University of California Los
       Angeles; 90024.
 SO    J Immunol. 1994 Feb 1;152(3):1197-205. Unique Identifier : AIDSLINE
       MED/94132615
 AB    The T2 mutant cell line is unable to load peptides into the MHC class I
       Ags inside the cells. These empty MHC class I Ags are not expressed on
       the cell surface unless the cells are cultured at low temperatures.
       Expression will occur at 37 degrees C only in the presence of peptides
       that bind to and stabilize the class I Ags. T2 cells transfected with
       the B*2705 gene were tested with a panel of anti-HLA-B27 mAb. Two of the
       antibodies, ME1 and KS3, reacted with the empty HLA-B27 expressed at low
       culture temperatures. Three antibodies, B27.M1, B27.M2, and Ye-2, were
       unreactive with these empty HLA-B27. The cells were then incubated with
       a panel of HLA-B27-binding peptides. One of the antibodies, Ye-2, became
       reactive when the cells were incubated with a peptide derived from HIV
       gp120 and to a less degree with a peptide derived from histone H3.3.
       Mouse L cells transfected with the B*2705 and the human beta 2m genes
       also reacted very poorly with B27.M1, B27.M2, and Ye-2. Those two
       peptides were also able to induce high increase in Ye-2 reactivity.
       Alternately, increase in Ye-2 reactivity was also observed when the L
       cells were incubated with IFN-gamma or TNF-alpha. These experiments
       indicate that the Ye-2 anti-HLA-B27 mAb recognizes HLA-B27 in the
       context of certain residing peptides either added exogenously or
       expressed endogenously. The B27.M1 and B27.M2 antibodies might share
       similar characteristics.
 DE    Amino Acid Sequence  Antigen-Presenting Cells/IMMUNOLOGY  Antigens,
       Viral/CHEMISTRY/IMMUNOLOGY  Cyclopentanes/PHARMACOLOGY  Heat-Shock
       Proteins/CHEMISTRY/IMMUNOLOGY  Histones/CHEMISTRY/IMMUNOLOGY  Human
       HLA-B27 Antigen/*IMMUNOLOGY  Interferon-gamma, Recombinant/IMMUNOLOGY
       Molecular Sequence Data  Peptides/CHEMISTRY/*IMMUNOLOGY  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Temperature  Tumor Necrosis
       Factor/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).