Document 0722
 DOCN  M9440722
 TI    Presence of HIV-1 in human parenchymal and non-parenchymal liver cells
       in vivo.
 DT    9404
 AU    Housset C; Lamas E; Courgnaud V; Boucher O; Girard PM; Marche C; Brechot
       C; INSERM U-370, Paris, France.
 SO    J Hepatol. 1993 Sep;19(2):252-8. Unique Identifier : AIDSLINE
       MED/94132538
 AB    The infection of liver cells by HIV-1 was investigated in vivo. Liver
       biopsies from 13 anti-HIV-1 antibody-positive patients were studied and
       HIV-1 DNA was revealed by polymerase chain reaction (PCR) in eight. In
       situ hybridization demonstrated the presence of HIV-1 RNA in all eight
       PCR-positive liver specimens. Mononuclear inflammatory cells in the
       portal tracts and Kupffer cells were labeled by a HIV-1 35S-RNA probe in
       all cases and by an anti-p24 monoclonal antibody, in seven cases. In
       addition, hepatocytes also clearly scored positive for HIV-1 RNA in
       three cases. These results demonstrate the infection of both parenchymal
       and non-parenchymal liver cells by HIV-1 in vivo and therefore show that
       HIV-1 can infect an epithelial CD4-negative cell type.
 DE    Acquired Immunodeficiency Syndrome/*PATHOLOGY  Base Sequence  Biopsy
       DNA, Viral/ANALYSIS/GENETICS  Human  HIV-1/GENETICS/*ISOLATION & PURIF
       Immunohistochemistry  In Situ Hybridization
       Liver/CHEMISTRY/*MICROBIOLOGY/*PATHOLOGY  Molecular Sequence Data
       Polymerase Chain Reaction  RNA, Viral/ANALYSIS/GENETICS  JOURNAL ARTICLE

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