Document 0734
 DOCN  M9440734
 TI    Identification of the microsporidian parasite, Enterocytozoon bieneusi
       in faecal samples and intestinal biopsies from an AIDS patient.
 DT    9404
 AU    Lumb R; Swift J; James C; Papanaoum K; Mukherjee T; Division of Clinical
       Microbiology, Institute of Medical and; Veterinary Science, Adelaide,
       South Australia.
 SO    Int J Parasitol. 1993 Sep;23(6):793-801. Unique Identifier : AIDSLINE
       MED/94131675
 AB    The microsporidian parasite, Enterocytozoon bieneusi, is currently
       recognized as a potentially important cause of chronic diarrhoea in
       patients infected with the human immunodeficiency virus (HIV). Faecal
       concentrates from a 38-year-old, HIV-seropositive patient examined by
       light and electron microscopy revealed the presence of numerous
       microsporidian spores. The structural characteristics of the spores were
       consistent with those previously described for Enterocytozoon bieneusi.
       Each spore contained a single nucleus, a posterior vacuole and a polar
       filament with 6-7 overlapping coils which appeared in cross-section as a
       series of 3 doublets. Mature spores were surrounded by an inner unit
       membrane, an electron-lucent endospore and a thin, electron-dense
       exospore. The identity of the parasite was confirmed by the detection of
       unique endogenous developmental stages in duodenal biopsies. Both
       proliferative and sporogonial plasmodia (meronts and sporonts) were
       observed and all stages were monokaryotic (single nucleus) and
       apansporoblastic (sporophorous vesicle absent). Proliferative and
       sporogonial plasmodia divided by plasmotomy and spore organelles (polar
       filament, attachment disc and polaroplast) were well developed prior to
       fission of the sporogonial plasmodium.
 DE    Adult  Animal  *AIDS-Related Opportunistic Infections  Case Report
       Feces/*PARASITOLOGY  Human  Intestines/*PARASITOLOGY  Male  Microspora
       Infections/*PARASITOLOGY  Microsporida/*ISOLATION & PURIF/ULTRASTRUCTURE
       Spores/ISOLATION & PURIF  JOURNAL ARTICLE

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