Document 0767
 DOCN  M9440767
 TI    A gene expression vector useful for protein purification and studies of
       protein-protein interaction.
 DT    9404
 AU    Zhao LJ; Narayan O; Marion Merrell Dow Foundation, Department of
       Microbiology,; Molecular Genetics and Immunology, University of Kansas
       Medical; Center, Kansas City 66160-7424.
 SO    Gene. 1993 Dec 31;137(2):345-6. Unique Identifier : AIDSLINE
       MED/94131309
 AB    This report describes the development of a gene expression vector that
       adds three features to the C-terminus of the putative synthesized
       protein: (1) a protein kinase A recognition domain allowing the protein
       to be radio-labeled in vitro, (2) an epitope marker for
       immunocharacterization of the protein with a commercial monoclonal
       antibody, and (3) a (His)6 block facilitating purification of the
       protein by metal chelating affinity chromatography. These features make
       it convenient to perform biochemical and functional analysis of the
       protein of interest.
 DE    Amino Acid Sequence  Antigenic Determinants/GENETICS  Base Sequence
       Chromatography, Affinity  Cyclic AMP-Dependent Protein
       Kinases/METABOLISM  DNA, Recombinant  Gene Products,
       nef/*GENETICS/METABOLISM  *Genetic Vectors  *HIV-1  Molecular Sequence
       Data  Polymerase Chain Reaction  Protein Binding/GENETICS  Recombinant
       Proteins/GENETICS/METABOLISM  Support, U.S. Gov't, P.H.S.  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).