Document 0781
 DOCN  M9440781
 TI    Localization of tumor-reactive lymph node lymphocytes in vivo using
       radiolabeled monoclonal antibody.
 DT    9404
 AU    Triozzi PL; Kim JA; Aldrich W; Young DC; Sampsel JW; Martin EW Jr;
       Department of Medicine, Ohio State University Comprehensive; Cancer
       Center/Arthur G. James Cancer Hospital and Research; Institute,
       Columbus.
 SO    Cancer. 1994 Feb 1;73(3):580-9. Unique Identifier : AIDSLINE
       MED/94130229
 AB    BACKGROUND. Lymph node lymphocytes vary in their responsiveness to
       tumor. A technique has been developed that uses radiolabeled monoclonal
       antibody (MoAb) against the tumor-associated mucin, TAG-72, and a
       gamma-detecting probe by which lymph nodes containing microscopic tumor
       and/or shed TAG-72 can be identified in vivo. The immunologic
       characteristics of these lymph nodes were examined. METHODS. Patients
       with colon cancer received 125I-labeled MoAb CC49 by intravenous
       injection preoperatively. During laparotomy lymph nodes that appeared
       normal on inspection and palpation but which contained radiolabeled MoAb
       were identified using a hand-held gamma-detecting probe. These lymph
       nodes and other lymph node and tumor specimens were resected for
       analysis. RESULTS. Lymph nodes identified by the probe were found by
       immunohistochemical studies to contain microscopic tumor and/or shed
       antigen associated with germinal centers. They were characterized by
       greater CD4+:CD8+ ratios, rates of expansion, and cytolytic activity
       compared with lymphocytes from lymph nodes with macroscopic tumor,
       noninvolved lymph nodes, and tumors. All lymph node lymphocytes
       identified by the probe demonstrated significant proliferative responses
       to autologous tumor and, in contrast to lymphocytes from noninvolved
       lymph nodes, significant proliferative responses to allogeneic TAG-72+
       tumor cells and to soluble TAG-72+ mucin. CONCLUSIONS. By locating lymph
       nodes with microscopic tumor and/or shed antigen, the use of
       radiolabeled MoAb in vivo can be used to reproducibly identify
       tumor-reactive lymph node lymphocytes. This technique may be useful in
       identifying cells for use in adoptive immunotherapy programs and in
       studying the regulation of immune responses in vivo.
 DE    Antibodies, Monoclonal  Antigens, Neoplasm/ANALYSIS/IMMUNOLOGY  Colonic
       Neoplasms/*IMMUNOLOGY/PATHOLOGY/THERAPY  Cytotoxicity, Immunologic
       CD4-CD8 Ratio  Glycoproteins/ANALYSIS/IMMUNOLOGY  Human  Immunotherapy,
       Adoptive  Iodine Radioisotopes  Lymph Nodes/IMMUNOLOGY/*PATHOLOGY
       Lymphocytes/*IMMUNOLOGY/PATHOLOGY  Support, Non-U.S. Gov't  Support,
       U.S. Gov't, P.H.S.  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).