Document 0786
 DOCN  M9440786
 TI    Lymphocyte subsets, sIL2-R and sICAM-1 in blood during allergen
       challenge tests in asthmatic children.
 DT    9404
 AU    Schmitt M; Niggemann B; Kleinau I; Nasert S; Kapp A; Wahn U; University
       Childrens Hospital (KAVH), Berlin, Germany.
 SO    Pediatr Allergy Immunol. 1993 Nov;4(4):208-13. Unique Identifier :
       AIDSLINE MED/94129696
 AB    In order to study cell activation in peripheral blood on bronchial
       allergen provocation up to 24 h, we investigated 32 asthmatic children,
       sensitive to house-dust mites. Six healthy young adult volunteers served
       as controls. Lymphocyte subsets (CD3, CD19, CD4, CD8) and activation
       markers (CD25-T, HLADR-T, CD23) in peripheral blood as well as soluble
       IL2-R and soluble ICAM-1 in serum were evaluated. In terms of clinical
       reaction, 23 children exhibited a DAR, 6 an EAR, 6 a LAR and 3 children
       did not show a bronchoconstrictor response to allergen challenge with
       house-dust mite extract (NAR). In comparison to controls, asthmatic
       children showed a significantly higher expression of CD23 on
       B-lymphocytes (p < 0.05). Other subsets were in the same range in both
       groups. After provocation there was a significant increase of
       CD4/CD8-ratio only in asthmatic children. Serum levels of sIL2-R were
       significantly higher in asthmatic children compared to controls at
       baseline as well as at 12 and 24 h after provocation, without variation
       during observation period. No differences were noted for sICAM-1. Our
       results confirm the hypothesis that lymphocytes, as important cells in
       regulation of allergic immune response, are recruited into peripheral
       blood under allergen challenge conditions in sensitized asthmatic
       children.
 DE    Adolescence  Adult  Allergens/*IMMUNOLOGY  Asthma/*IMMUNOLOGY  Cell
       Adhesion Molecules/*BLOOD  Child  CD4-CD8 Ratio  Female  Human
       Lymphocyte Subsets/*IMMUNOLOGY  Male  Receptors, Interleukin-2/*ANALYSIS
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).