Document 0841
 DOCN  M9440841
 TI    Oral immunization with recombinant BCG induces cellular and humoral
       immune responses against the foreign antigen.
 DT    9404
 AU    Lagranderie M; Murray A; Gicquel B; Leclerc C; Gheorghiu M; Laboratoire
       du BCG, Institut Pasteur de Paris, France.
 SO    Vaccine. 1993 Oct;11(13):1283-90. Unique Identifier : AIDSLINE
       MED/94127077
 AB    It has been shown recently that BCG can be used as a live recombinant
       vaccine to stimulate immune responses. Proliferative or cytotoxic T-cell
       responses against several viral proteins such as HIV Gag, Env or Nef
       were obtained after parenteral immunization with BCG expressing these
       proteins. Antibody responses were also obtained after immunization of
       mice with recombinant BCG strain which expressed lac Z under the control
       of a promoter sequence isolated from Mycobacterium paratuberculosis. We
       have used this recombinant vaccine in guinea-pigs to investigate the
       influence of various routes of immunization on the immunogenicity of a
       foreign antigen expressed by recombinant BCG. Guinea-pigs were immunized
       by oral, respiratory or intradermal routes and proliferative responses,
       delayed-type hypersensitivity and antibody responses specific for
       beta-galactosidase were followed for 16 weeks. Results demonstrated that
       humoral and cellular immune responses specific for beta-galactosidase
       can be produced in all groups of guinea-pigs. However, the respiratory
       and especially the oral route of administration induced higher local and
       systemic immune responses than the intradermal route of immunization.
       Moreover, the oral immunization of mice with this recombinant BCG
       induced IgA responses which could be detected in both sera and
       intestinal secretions. Therefore, this study demonstrates for the first
       time that oral immunization with recombinant BCG can induce strong
       cellular and humoral immune responses.
 DE    beta-Galactosidase/PHARMACOLOGY  Administration, Inhalation
       Administration, Oral  Animal  Antibody Formation/*DRUG EFFECTS
       Antigens/*PHARMACOLOGY  BCG Vaccine/*ADMINISTRATION &
       DOSAGE/*PHARMACOLOGY/  PHARMACOKINETICS  Comparative Study  Drug
       Stability  Female  Guinea Pigs  Hypersensitivity, Delayed/IMMUNOLOGY
       Immunity, Cellular/*DRUG EFFECTS  Immunization  Lymphocyte
       Transformation/DRUG EFFECTS/IMMUNOLOGY  Male  Mice  Mice, Inbred BALB C
       T-Lymphocytes/DRUG EFFECTS/IMMUNOLOGY  Tissue Distribution  Tumor
       Necrosis Factor/METABOLISM  Vaccines, Synthetic/*ADMINISTRATION &
       DOSAGE/METABOLISM/  *PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).