Document 0854
 DOCN  M9440854
 TI    [Cryptosporidium parvum in patients with chronic diarrhea and AIDS:
       diagnosis by means of indirect immunofluorescence with monoclonal
       antibodies]
 DT    9404
 AU    Weitz JC; Astorga B; Laboratorio de Referencia de Parasitologia,
       Instituto de Salud; Publica, Santiago de Chile.
 SO    Rev Med Chil. 1993 Aug;121(8):923-6. Unique Identifier : AIDSLINE
       MED/94126602
 AB    The diagnosis of cryptosporidiosis is difficult when oocyst elimination
       is poor as occurs in AIDS patients. Aiming to improve the diagnosis, 144
       fecal samples coming from AIDS patients with diarrhea, were studied
       using indirect immunofluorescence with anti-Cryptosporidium monoclonal
       antibodies. The results were compared with Ziehl Neelsen and safranine
       stainings. Twenty three samples (15.9%) were positive for
       Cryptosporidium with at least one of the three methods. Sensitivities
       were 78.3% for immunofluorescence, 86.9% for Ziehl Neelsen and 91.3 for
       safranine stainings. The specificity of the three methods was 100%. It
       is concluded that immunofluorescence does not improve the diagnostic
       accuracy of cryptosporidiosis and its high cost precludes its use in
       routine laboratories.
 DE    Animal  Antibodies, Monoclonal/DIAGNOSTIC USE  Antibodies,
       Protozoan/*ANALYSIS  AIDS-Related Opportunistic Infections/*DIAGNOSIS
       Chronic Disease  Comparative Study  Cryptosporidiosis/*DIAGNOSIS
       Cryptosporidium parvum/*IMMUNOLOGY  Diarrhea/*DIAGNOSIS  Double-Blind
       Method  English Abstract  Feces/PARASITOLOGY  Fluorescent Antibody
       Technique  Human  Prospective Studies  Sensitivity and Specificity
       CLINICAL TRIAL  JOURNAL ARTICLE

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